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Fluid Inclusions and Metallogenic Conditions of the Dashuigou Tellurium Deposit, Tibet Plateau, Southwest China

DOI: 10.31038/GEMS.2021331

Abstract

By thoroughly researching the microscopic characteristics and compositions of fluid inclusions in various minerals of the Dashuigou independent tellurium deposit in Southwest China, the authors of this paper uncover metallogenic conditions of the only independent tellurium deposit in the world. The principal compositions of the metallogenic hydrothermal fluids are Na+, K+, Ca2+, Mg2+, SO42-, Cl, F, H2O, CO2, CH4, H2, N2, CO and C2H6. The salinity of fluid inclusions within the metallogenic epochs varies between 13.8%-36.2%, which falls into a medium-high salinity range. The salinity of quartz samples associated with tellurides formed during the tellurium epoch is 14.9%-18.7%, which is within the medium salinity range. Metallogenic pressure is calculated at 0.647-1.020 Kbar, and the corresponding mineralization depth is 4.08-2.16 km. Mineralization temperatures of the deposit’s early and late metallogenic epochs are respectively 336.0-406.0 and 216.9-229.0°C. The metallogenic hydrothermal solutions are SO42- – Ca2+ type, or SO42- – Na+ – K+ – Ca2+ type, especially during the early Pyritic Epoch, and Na+-K+-Cl-SO42- type during the late metallogenic epoch. The metallogenic hydrothermal solutions of the deposit are of moderate salinity, mesothermal and mesogenetic.

Keywords

Metallogenic condition; Fluid inclusion; Homogenization temperature; Decrepitation temperature; Metallogenic epoch; The Dashuigou tellurium deposit; Tibet Plateau

Introduction

Tellurium (Te) is usually categorized as a scattered or dispersed element (abbreviated as SM). SM are those metals, semimetals and/or nonmetals that have similar geochemical characteristics with Clark values too low to enrich into independent deposits, but that play very important roles in modern science, industry, national defense and at the frontiers of technology. It is thought in the traditional theory of mineral deposits and geochemistry that Te could not form independent deposits, but only exist as associated components in other metallic deposits. The abundance of Te in the Earth’s Crust is very low. According to Li [1], the average content of Te in the Earth’s crust is 2.0 x 10-8 in China, and only 1.34 x 10-9 worldwide. At present, the world’s supply of refined tellurium is mainly recovered from Te-bearing minerals including pyrite, sphalerite, chalcopyrite, galena, pyrrhotite, volcanogenetic sulfur, bismuthinite, arsenopyrite, and cassiterite, etc. Generally speaking, only sulfide ores containing more than 0.002% Te can be used. As a result, the amount of refined tellurium that can be recovered is very limited. Most of the recoverable Te in the world is from copper deposits, and it is estimated that only 0.065 kg of Te can be produced in the refining process of one ton of copper [2,3]. The Dashuigou tellurium deposit is the only independent tellurium deposit in the world. Since its discovery in 1992, it has aroused widespread concern from domestic geologists. Chen [4] believed that tellurium mineralization is related to Yanshanian alkaline intrusive rocks, while Luo [5,6] believed that the mineralization is related to Yanshanian granitic magma activity. Yin [3,7,8] proposed that scattered elements including tellurium and bismuth originated from gas blown off from the deep Earth and enriched through nano-effect. Wang [9] summarized the metallogenic process of the Dashuigou tellurium deposit as follows: a volcanic eruption deposit was formed on the ancient seafloor with magmatic eruption in the late Proterozoic. Then, the deposit was strongly superimposed and reconstructed by the Mesozoic multistage regional metamorphic hydrothermal activities.

Regional Geology

The Dashuigou tellurium deposit is located in the transitional belt between the Yangtze Platform and Songpan-Ganzi folded belt, as part of the Tibetan Plateau (Figure 1). The deposit is nestled in the convergence between the Indian, Eurasian and Pacific Plates. The crust-mantle structures and properties in the region are the result of tectogenesis through various geological times. It implies the turning boundary of the Earth’s crust’s thickness. It is also a gravity gradient zone which controls not only the production and development of earthquakes and tectonomagmatic events, but also the distribution of a series of mineral deposits. Geophysical data indicates that the upper mantle below the region uplifts obviously. As a result, the area possesses high heat flow geophysical characteristics [3,5,6,10,11].

fig 1

Figure 1: Regional geology (after Yin, 1996).

1. The lower and middle Triassic metamorphic rocks; 2. The Permian metamorphic rocks; 3. The Devonian metamorphic rocks; 4. Metamorphic rocks of the Sinian system; 5. Metamorphic base complex of the Archean Kangding group; 6. Plutonic granite of the Indosinian orogeny; 7. Plutonic alkaline syenite of the Indosinian orogeny; 8. Plutonic monzonitic granite of the Indosinian orogeny; 9. Hypabyssal sillite of the Indosinian orogeny; 10. The late Hercynian basic-ultrabasic rocks; 11. The late Proterozoic plutonic granite; 12. The early Proterozoic-Archean plutonic quartz diorite; 13. The deep and large fault; 14. The geological boundary; 15. Village and/or town; 16. The tellurium deposit. There is also a low-velocity, low resistivity zone in the middle crust that is interpreted as a decollement. The abnormal mantle exists under the crust in the region. It has properties of both geosyncline and platform, as well as its own special characteristics. The belt is a geo-tectonically active zone with very complicated igneous rock structures. According to the regional geophysical data, the region’s characteristics exhibit high velocity, high density, high resistance, high geothermal flow, high magnetism as well as well-developed earthquakes and mantle’s uplift. In summary, this region is both geologically very active and a very important south-north trending tectonomagmatic-mineral belt [3-6]. The strata, igneous rocks and structures trend south-northward. The strata are low-grade metamorphic rocks of the Silurian, Devonian, Permian systems and middle-lower Triassic series. A large amount of Archaean metamorphic rocks of the Kangding Group emerge to the southeast of the deposit. The well-developed igneous rocks in the region include ultrabasic, basic, neutral, acid and alkaline, produced in different geological times. Different types of mineral resources in the region are very rich; many of these are well known, including Ti, V, Cu, Pb, Zn, SM, REE, coal, asbestos and the Panzhihua Vanadium Titano-magnetite deposit [3-6].

Mine Geology

The strata of the area are low-grade metamorphic rocks of the lower-middle Triassic age, including marble, slate and schist. The main wall-rocks of the ore bodies are schist and slate. All of the Triassic strata make up a NNE-trending dome. The geological and geochemical characteristics in the area indicate that the protolith of the tellurium ore veins’ direct wall-rocks is poorly differentiated mantle-derived basalt (Figure 2).

fig 2

Figure 2: Mine geology (after Yin, 1996).

Both faults and folds are well-developed in the area. The annular and linear structures together make up special “Ø” pattern structures, which control the formation of different types of endogenetic mineral deposits, including the Dashuigou tellurium deposit. No intrusive rocks emerge within a 5 km radius around the deposit. Only two small Permian ultrabasic-basic rock bodies emerge within a 10 km range of the deposit. Large neutral, acid and alkaline intrusive bodies exist beyond 10 km, which are unrelated to the deposit (Figure 1). Quantitative chemical analyses of Te, Bi, Se, As, Au, Ag, Cu, Pb and Zn were conducted on different rock samples including granites, metamorphic rocks, altered rocks, and carbonate veins of different geological times. The main findings are summarized below [3,12-15]. The Te content in the granites is under 1 x 10-7, which is similar to its Clark value in the Earth’s crust. Te in the metamorphic rocks is slightly higher than in the granites and varies slightly between metamorphic rocks of different geological times, while being relatively higher in the Triassic metamorphic rocks. Of the metamorphic rocks in the same geological time, the Te content in the slate and schist is higher than in the marble. Te content in rocks of the same stratohorizon of the same geological time also varies; namely, it is higher in rocks within the mining area than in those beyond the mining area. Te content is closely related to the intensity of alterations; that is, the ore-forming elements are not derived from the country rocks, but instead from the mantle. The deposit is located at the northeastern end of the Triassic metamorphic dome. The ore bodies are controlled by and fill a group of shear fractures. Ten tellurium ore veins have been discovered, which strike from 350 to 10 degrees and dip at 55 to 70 degrees westward. Widths of the ore bodies vary between 25 and 30 cm. The narrow ore bodies are in the shape of lenticular veins and have sharp contact with the wall rocks (Figures 3 and 4).

fig 3

Figure 3: Horizontal projection of the telluride veins of the Dashuigou deposit (after Yin, 1996).

fig 4

Figure 4: Longitudinal section the telluride and pyrrhotite veins of the Dashuigou deposit (after Yin, 1996).

The altered rocks occur in narrow bands ranging between several centimeters and one meter in thickness. Altered zones beside the massive ore veins are narrower, at only several centimeters wide. The dominant alterations include dolomitization, silicification, biotitization, muscovitizaion, tourmalinization, sericitizaion, greisenization, and chloritization [3,7,14-20]. Approximately thirty minerals are identified in the ore, which include tetradymite, pyrrhotite, pyrite, dolomite, quartz, chalcopyrite, tsumoite (BiTe), tellurobismuthite (Bi2Te3), galena, magnetite, gold, silver, electrum, ilmenite, calcite, calaverite, siderite, mannesite, rutile, muscovite, biotite, sericite, hornblende, chlorite, plagioclase, K-feldspar, tourmaline, hematite, garnet, apatite, and epidote. The first five minerals are the most important and comprise 85% of the ore, though generally tetradymite is so rare that many monographs on mineralogy do not have any related data on it [3,10,19-22]. Replacement, remnant, reaction border, and granular are the dominating textures of the ore. Massive, vein/veinlet, stockwork veins are the dominating structures of the deposit (Figures 5 and 6).

fig 5

Figure 5: Lead grey-silvery colored tetradymite ± tsumoite (BiTe) ± tellurobismuthite (Bi2Te3) fine veinlets in massive pyrrhotite (dark colored background) + dolomite (brownish white) from the deposit (sample #: SD40, Ore body #I-1 in Drift 3).

fig 6

Figure 6: Lead grey-silvery colored tetradymite ± tsumoite (BiTe) ± tellurobismuthite (Bi2Te3) fine veinlets in massive pyrrhotite (dark colored) + dolomite (brownish white) + wall rock (dark brown) from the deposit (sample #: SD34, Ore body #I-1 in Drift 3).

The most important ores are massive and the secondary ores are disseminated. The Te content in the ore varies between 0.01% and 34.58%.

Two mineralization epochs and five stages exist in the deposit [3,8,23]:

  • Pyrrhotite epoch (177.7~165.1 Ma): including three mineralization stages: carbonate stage (I) → pyrrhotite stage (II) → chalcopyrite stage (III) (from early to late).
  • Tellurium epoch (91.71~80.19 Ma): including two mineralization stages, namely: tetradymite stage (I) → tsumoite (BiTe0.97) stage (II).

Mineralization Epoch and Mineral Sequence

Based on the mutually crosscutting relationships of various veins/veinlets in the deposit, including those of pyrrhotite, chalcopyrite, tetradymite, tsumoite, dolomite and quartz, in addition to the features of microscopic texture and structure between gangue and ore minerals, the mineralization epochs and stages as well as mineral sequence are summarized in Figure 7 [3,8,23].

fig 7

Figure 7: Mineralization epochs & stages and mineral sequence of the deposit.
Note: * – mineral inclusion homogenization temperature (dolomite for carbonate stage, quartz for tsumoite stage), please refer to Tables 1 and 2 for more detailed information; ** – mineral inclusion decrepitation temperatures of the corresponding stages (that of the pyrrhotite stage is the average of decrepitation temperatures of both the pyrite and pyrrhotite of the pyrrhotite stage.

Table 1: Decrepitation temperatures of fluid inclusion in the minerals from the deposit.

table 1

Table 2a: Characteristics of fluid inclusions in the minerals from the deposit.

table 2a

Table 2b: Characteristics of fluid inclusions in the minerals from the deposit (cont’d).

table 2b

Carbonate Stage

A large quantity of iron-dolomite, quartz and lesser calcite veins/veinlets occurred within this stage, which are brown and/or yellowish brown broken coarse-grained due to iron staining (Figures 5 and 6). Decrepitation temperatures of the fluid inclusions in the dolomite, one of the dominant minerals of this stage, are listed in the corresponding table of this paper.

Pyrrhotite Stage

The largest quantity of pyrrhotite formed during this stage. Some coarse- to very coarse-grained pentagonal pyrite can be seen in the anhedral crystals of pyrrhotite. Decrepitation temperatures of the fluid inclusions in both the pyrrhotite, one of the dominant minerals, and pyrite of this stage are listed in the corresponding table of this paper.

Chalcopyrite Stage

Many chalcopyrite veins/veinlets formed within this stage, filling in fractures of pyrrhotite and/or crosscutting pyrrhotite. Part of the chalcopyrite is within telluride minerals in the vermicular form. Decrepitation temperatures of the fluid inclusions in the chalcopyrite, the dominant mineral of this stage, are listed in the corresponding table of this paper.

The three prior mineralization stages consist of the early metallogenic epoch of the deposit, the pyritic epoch (177.7-165.1 Ma) of the early Yanshan orogeny. The following two mineralization stages consist of the tellurium metallogenic epoch (91.71-80.19 Ma) of the late Yanshan orogeny [3,24].

Telluride Stage

Large quantities of massive/semi-massive telluride veins formed during this stage, associated with clean, milky-white quartz, dolomite, calcite, muscovite/sericite and native gold. Decrepitation temperatures of the fluid inclusions in the tetradymite, the dominant mineral, and part of the associated dolomite of this stage are listed in the corresponding table of this paper.

Tsumoite Stage

Tsumoite and chalcopyrite together consist of emulsion droplets and/or vermicular immixing of solid solution texture at the contacts between tetradymite and pyrrhotite (Figures 5, 6 and 8).

fig 8

Figure 8: 1~3: Reflection color and their mutual relationships between tsumoite (bright white), tetradymite (white), and pyrrhotite (grey) in the back scattered electron (composition) image from the deposit (thin section (-) (Note: 1 (×120), 2 (×540), & 3 (×200)). 4: Te Kα X ray image indicating chemical composition distributions of telluride including tetradymite and tsumoite (white): the denser the white spots, the higher the Te content; the black colored background is pyrrhotite from the deposit (×400).

The mineralization temperatures of this stage shown in Figure 7 are based on the homogenization temperatures of the fluid inclusions in the quartz associated with tsumoite. Please refer to the corresponding table of this paper for more detailed information.

Sampling and Analytical Methodology

Field Sampling

Samples were collected from proper locations of the deposit’s typical ore bodies and wall rocks in the study area. They were described in detail on site, then properly labeled or numbered and wrapped with waxed paper to avoid cross contamination with other samples in the same sample box, and finally packed and shipped to the work laboratory.

Lab Sampling and Preliminary Processing

In the lab, all samples were sorted, air-dried, stage crushed to 6-Tyler mesh, well homogenized, and then rotary split into 1 kg assay aliquots. One assay aliquot was wet screened into different sized fractions, and the -10 mesh+65 mesh fraction was used for this study. After being crushed and screened through different sizes of mesh, the corresponding minerals in fraction from 10 to 65 mesh to be used for further analyses were then manually separated and picked up under binocular microscope, and their crystal forms and other physical mineralogical characteristics were observed and described in detail. Next, the selected mineral samples were pulverized to 90% passing 75 μm for further research and testing, in order to help reveal the mineralization mechanism of the corresponding deposit.

Analytical Methodology

The gaseous components of the fluid inclusions in quartz were analyzed via both the Raman spectroscopy and gas chromatographic methods. The Raman spectroscopy technique has a wide field of applications, ranging from qualitative detection of solid, liquid and gaseous components to identification of polyatomic ions in solution. It is also a versatile non-destructive technique for fluid inclusion analysis and is commonly used to calculate the density of CO2 fluids, the chemistry of aqueous fluids, and the molar proportions of gaseous mixtures present as inclusions… The main advantages of this technique are the minimal sample preparation required and its high versatility. The particle size of the 99.9% purity mineral samples was controlled to 0.5-1.0 mm to avoid damaging the fluid inclusions. According to their respective burst temperatures, the primary inclusions in the minerals were opened by thermal explosion, and the fluid components obtained by heat-blast-leaching. The released gas-phase components such as H2O, CO2, CO, H2, N2, and CH4 were measured by gas chromatography. Decrepitated mineral was added with deionized water, and ultrasonic extraction was then conducted. The extract liquor was measured by atomic absorption spectrometer for K+, Na+, Ca2+, Mg2+ and other cationic components in the solution, while anion components such as F, Cl, SO42-, etc. were determined through ion chromatography or spectrophotometry. In addition, Fe, Cu, Pb, Zn, Sb, Hg, Au, Ag and other related ore-forming metal elements can be determined by the atomic absorption flameless method as required. Then, the gas and liquid phase components in the mineral inclusions were converted into the mass concentration of each component contained in the mineral inclusion aqueous solution. Conversion of liquid components: At room temperature, 1 ml of water weighs about 1 gram. First, the mass of water in the analysis result is converted into the volume VH2O, and the mass concentration ρB of each ion in the inclusion water is calculated via the following formula [25,26]:

The volume of inclusion water VH2O = ωH2O/1000. Mass concentration of ions in liter of water ρB = ωB/VH2O.

For the measurement data of vapor phase components H2O, CO2, CO, H2, and CH4, a relevant diagram [27] was applied to estimate the temperature, pressure, oxygen fugacity, carbon dioxide fugacity, reduction coefficient, and other geochemical parameters such as pH and Eh of the equilibrium among the components in the mineral inclusions.

Characteristics of the Fluid Inclusions

General Characteristics

The fluid inclusion characteristics of part of the deposit’s gangue and ore minerals are summarized in Tables 2 and 3 and discussed as follows. Fluid inclusions are well developed in most of the deposit’s minerals, but their characteristics differ from each other within different host minerals. Dolomite usually has less liquid inclusions than both calcite and quartz. The sizes of fluid inclusions in dolomite and calcite are usually between 3-5 µm, which is smaller than those in quartz, which is usually 5-10 µm (Table 2). Fluid inclusions in both dolomite and calcite are mainly liquid and usually scattered without any orientation in mineral distribution, with a gas-liquid ratio of less than 5%. Those in quartz, which are mainly gaseous-liquid inclusions within the deposit, are more complicated than those in both dolomite and quartz; either scattered without any orientation in distribution, or in zonal distribution in certain orientations (Figure 6). Fluid inclusions in the tourmaline-quartz vein collected from the periphery of the deposit are mainly gaseous-liquid inclusions and differ from those of quartz within the deposit; a few 3 phase inclusions could be seen in the quartz vein collected around the deposit.

Fluid inclusions in both dolomite and calcite are usually oval and/or polygon in shape, while those in quartz are either oval, polygon, irregular, strip and/or tubular. A few perfect cubic NaCl crystals could be seen in several of the fluid inclusions in quartz (Figure 9).

fig 9

Figure 9: Fluid inclusions in quartz and calcite of the deposit. A: gaseous-liquid inclusions in quartz from #I-4 Ore Body (X 400), B: gaseous inclusions in quartz from #I-4 Ore Body (X 400), C: cubic NaCl crystal in gaseous-liquid inclusions in quartz from #I-7 Ore Body (X 400), D: gaseous-liquid inclusions in calcite from coarse grained marble of the lower Triassic strata of the deposit (X 400).

Chemical Compositions

Chemical compositions of the fluid inclusions of part of the gangue and ore minerals are listed in Table 3. It can be seen from the table that cations in the fluid inclusions include Na+, K+, Ca2+ and Mg2+, while anions include SO42-, Cl and F, and the major gaseous compositions include H2O and CO2, with smaller CH4, H2, N2, CO and C2H6. Na+/K+ ratios of the fluid inclusions of the region’s minerals are either above or below 1.0, meaning that compositions of the hydrothermal solutions are not homogeneous. Ca2+/ Mg2+ ratios of the fluid inclusions are all above 1.0, meaning that the metallogenic solutions are rich in Ca2+ but poor in Mg2+. Both SO42-/Cl and Cl/F ratios are over 1.0, meaning that the metallogenic solutions are richest in SO42-, moderate in Cl, but poor in F. Most of the samples had H2O/ CO2 ratios of over 1.0, meaning the solutions are richer in H2O than in CO2. (CO2 + H2 + CH4)/N2 values of all samples are over 1.0, showing that the hydrothermal solutions are rich in CO2, H2 and CH4.

Table 3a: Chemical compositions of the gaseous-liquid fluid inclusions in the minerals.

table 3a

Table 3b: Chemical compositions of the fluid inclusions in the minerals (cont’d).

table 3b

Table 3c: Chemical compositions of the fluid inclusions in the minerals (cont’d).

table 3c

Discussion

Previous research on the ore-forming fluid of the Dashuigou tellurium deposit is roughly divided into the magma hydrothermal theory [4-6,10,11,26,28,29], the metamorphic hydrothermal theory [30,31], and the mixed hydrothermal theory [5,9,30] obtained the study of gas-liquid inclusions and found that the ore-forming temperature of the Dashuigou tellurium deposit varied between 350 and 120°C. The salinity is 7.2 wt% ~ 35 wt% NaCl, of which the salinity value in the early and late quartz veins can be as high as 20 wt% to 35 wt% NaCl, the oxygen fugacity is fO2 = -42.26 ~ -45.49, and the ore-forming hydrothermal fluid is weakly acidic and neutral (pH = 6.32 ~ 6.29). The CO2/H2O ratio in the quartz is 0.137 ~ 0.208, and the CH4 content is 12.61 ~ 24.85 ml/100 g. Based on uniform temprature measurement of the gas-liquid inclusions in the deposit’s dolomite and quartz, Li [29] and Cao [10-11] concluded that the uniform temperature of each mineralization stage is roughly similar: 221 ~ 259°C, average 239°C for Stage I; 215 ~ 256°C, average 235°C for Stage II; 192 ~ 243°C, average 224°C for Stage III. The corresponding metallogenic pressure is 823.684 × 105 Pa ~ 965.409 × 105 Pa, with an average of 884.156 × 105 Pa. Both CO2 and H2O-CO2 inclusions are abundant, reflecting that mineralization occurs under relatively closed conditions. The metallogenic depth estimated by the static rock pressure model is 3,339 m, which is equivalent to the thickness of the measured overlying strata (3650 m). Since the characteristics of inclusion types in each mineralization stage are not substantially different, they are considered to have similar metallogenic depths. According to the ore-forming pressure and salinity, the temperature correction value was found to be about 80°C, so the metallogenic temperatures of stages I, II, and III were 319°C, 315°C, and 304°C, respectively. The salinity of ores from this deposit varies widely, with NaCl content ranging from 7.17% to 33.27%. Among them, the rock salt gas-liquid water inclusions have the highest salinity, while the gas-liquid water inclusions and liquid-liquid H2O-CO2 inclusions have lower salinity and are basically the same. The salinity of fluids in each mineralization stage is roughly similar, where the NaCl content in stage I is 16.5% ~ 30.83%, with an average of 21.21%; stage II is 17.66% ~ 33.27%, with an average of 25.77%; stage III is 7.17% ~ 32.34%, with an average of 17.55%. The gas phase composition is mainly H2O, followed by CO2, and containing a small amount of H2, CO and CH4. According to observation under a microscope, the fluid inclusions in the minerals from this deposit are mainly H2O-CO2 and CO2. The fO2 of the mineralization fluid is: stage Ⅰ 10.0 × 105 ~ 34. 0 × 105 Pa, stage Ⅱ10.0 × 105 ~ 34.8 × 105 Pa, stage Ⅲ10.0 × 105 ~ 35.6 × 10 5Pa. The pH value of the hydrothermal solution during the tellurium mineralization stage is 5.91 ~ 5.87. Through study of gas-liquid inclusions from the same deposit, Chen [28] concluded that the inclusions are rich in CO2, CO2-H2O (low salinity fluid), and CO2-H2O-NaCl (high salinity fluid); namely, three systems of fluid inclusions exist in the deposit, of which tellurium mineralization is related to the first two systems. Most of the inclusions leak and/or burst before being homogenized. The metallogenic temperature of the pyrrhotite stage is around 500°C and that of the telluride stage is 400°C. The fluid density changes between 1.04 ~ 0.76 g/cm3, and the metallogenic pressures are respectively 450 ~ 500 MPa and 240 ~ 300 MPa. Mineralization occurs under high temperature and high pressure. The results of this paper are as follows: fluid inclusion homogenization temperatures vary greatly (Table 2): those of quartz formed in pre-tellurium mineralization and collected outside the deposit are between 339.0 and 369.4°C. These samples include series #1, 2, 4 and 5 in Table 2. Other quartz samples in Table 2 are associated with telluride veins and their fluid inclusion homogenization temperatures should be at typical values when tellurium ore bodies emplaced, or between 180.0-250.0°C. This range includes part of the decrepitation temperatures of those minerals formed during the tellurium epoch mentioned earlier in this paper. The salinity of fluid inclusions within the whole metallogenic epochs varies between 13.8%-36.2%, which falls into a medium-high salinity range. That of minerals formed during the tellurium epoch, for instance three quartz samples associated with tellurides and collected from #I-4 Ore Vein in Table 2, is between 14.9%-18.7%, well within the medium salinity range. The formation pressures of mineral fluid inclusions correlate positively with homogenization temperatures (Table 2). Pressures of the series #6 through #9 samples in the table, acquired from minerals formed during tellurium mineralization, vary between 0.647-1.02 Kbar. This should be the pressure at which point telluride veins emplaced in the deposit. The corresponding mineralization depth is 4.08-2.16 km, while mineralization temperatures of the early and late metallogenic epochs of the deposit are respectively 336.0-406.0 and 216.9-229.0°C. Fluid inclusion compositions in both pyrrhotite and pyrite that formed in the same mineralization stage of the metallogenic epoch are similar to each other. Those of fluid inclusions in quartz samples collected from the deposit are also alike. Compositions of fluid inclusions of dolomite and calcite have both similarities and differences, especially those of dolomite and calcite collected from outside the deposit compared to those collected within the deposit. Differences also exist between dolomite from wall-rock marble and from dolomite veins. This may mean that compositions of fluid inclusions vary from one host mineral to another, and minerals formed in different environments or across different geological events have different fluid inclusion compositions. The metallogenic hydrothermal solutions, which are not homogeneous, are richest in SO42-, richer in H2O than in CO2, rich in Ca2+, CO2, H2, and CH4, moderate in Cl, but poor in Mg2+ and F.

Conclusion

Based on the discussions above, preliminary conclusions of the Dashuigou independent tellurium mine’s metallogenic conditions are summarized as follows: The metallogenic hydrothermal solutions are SO42- – Ca2+ type, or SO42- – Na+ – K+ – Ca2+ type, especially during the early metallogenic Pyritic Epoch, and Na+ – K+ – ClSO42- type during the late metallogenic epoch. The hydrothermal solutions of the deposit are mesothermal and of moderate salinity, as well as mesogenetic. Principal compositions of the metallogenic hydrothermal fluids are Na+, K+, – Ca2+, Mg2+, SO42-, Cl, F H2O, CO2, CH4, H2, N2, CO and C2H6; Salinity of the fluid inclusions within the metallogenic epochs varies between 13.8%-36.2%, which falls into the medium-high salinity range; that of minerals formed during tellurium epoch, for instance, three quartz samples associated with tellurides and collected from #I-4 Ore Vein in Table 2, is 14.9%-18.7%, which is within the medium salinity range. Metallogenic pressure is between 0.647-1.020 Kbar and the corresponding mineralization depth is 4.08-2.16 km. Mineralization temperatures of the early and late metallogenic epochs of the deposit are respectively 336.0-406.0 and 216.9-229.0°C.

Acknowledgement

Support for this study was received from the China National Postdoctoral Foundation, Orient Resources Ltd., and Bureau Veritas Commodities Canada Ltd. Additional support was provided respectively by Prof. R. Pei of the Chinese Academy of Geological Sciences, also an Academician of the Chinese Academy of Engineering, Prof. Y. Zhai of China University of Geosciences in Beijing, also an Academician of the Chinese Academy of Sciences, Prof. J. Zhou of the Chinese Academy of Geological Sciences, and Prof. Y. Zhang of the College of Earth Sciences, Jilin University in Changchun of China, all of whom provided insightful discussions and critical reviews of this manuscript. All measurements and chemical analyses, including the back scattered electron (composition) and Te Kα X ray images of the thin section from the deposit, included in this article were carried out in the labs of the Chinese Academy of Geological Sciences in Beijing. The authors very much appreciate the time invested respectively by D. S. Yin and S. Daly, for their review and editorial work on this contribution.

Data Availability

The data that support the findings of this study is available from the authors upon reasonable request; see authors’ contributions for specific data sets below.

Contributions

The whole research included in the paper was proposed and done by the first author J. Yin. The chemical compositions of fluid inclusions were sorted and researched by the second author Hongyun Shi of this paper. Both authors prepared and reviewed the manuscript and approved the final version of the manuscript. Author Contributions. Conceptualization, JIANZHAO YIN; Investigation, JIANZHAO YIN and Hongyun Shi; Project administration, JIANZHAO YIN; Writing – original draft, JIANZHAO YIN.

Competing Interests

The authors declare no competing interests.

References

  1. Li T (1976) Abundance of chemical elements in the Earth. Geochemistry 3: 167-174.
  2. Yin, JZ, Chen, YC, Zhou, JX (1995) Introduction of tellurium resources in the world. Journal of Hebei College of Geology 18: 348-354.
  3. Yin, JZ (1996) On the metallogenic model and mineralizing mechanism of the Dashuigou independent tellurium deposit in Shimian County, Sichuan Province, China-the first and only independent tellurium deposit in the world. Chongqing: Chongqing Publishing House, 190pp.
  4. Chen YC, Mao JW, Luo YN, Wei JX, Cao, ZM. (1996) Geology and Geochemistry of the Dashuigou tellurium (gold) deposit in Western Sichuan, China. Beijing: Atomic Energy Press 146pp
  5. Luo, YN, Fu DM, Zhou, SD (1994) Genesis of the Dashuigou tellurium deposit in Sichuan Province of China. Bulletin of Sichuan Geology 14: 101-110.
  6. Luo YN, Cao ZM, Wen, CQ (1996) Geology of the Dashuigou independent tellurium deposit. Chengdu: Southwest Communication University Publishing House 30-45.
  7. Yin JZ (1996) The metallogenic model and mineralizing mechanism of the Dashuigou independent tellurium deposit in Shimian County, Sichuan – the first and only independent tellurium deposit in the world. Acta Geoscientia Sinica special issue 93-97.
  8. Yin JZ, Shi HY (2019) Nano effect mineralization of rare elements–taking the Dashuigou tellurium deposit, Tibet Plateau, Southwest China as the example. Academia Journal of Scientific Research 7: 635-642.
  9. Wang RC, Lu, JJ, Chen XM (2000) Genesis of the Dashuigou tellurium deposit in Sichuan Province, China. Bulletin of Mineralogy, Petrology and Geochemistry 4: 348~349.
  10. Cao ZM, Luo YN (1994) Mineral sequence and ore genesis of the Sichuan telluride lode deposit in China. In: New Research Progresses of the Mineralogy, Petrology and Geochemistry in China. Lanzhou: Lanzhou University Publishing House pg: 476-478.
  11. Cao ZM, Wen CQ, Li BH (1995) Genesis of the Dashuigou tellurium deposit in Sichuan Province of China. Science China (B) 25: 647-654.
  12. Yin, JZ, Zhou JX, Yang BC (1994) Geological characteristics of the Dashuigou tellurium deposit in Sichuan Province, China. Earth Science Frontiers 1: 241-243.
  13. Yin, JZ, Chen, YC and Zhou, JX (1995) Original rock of the host rock of the Dashuigou independent tellurium deposit in Sichuan Province, China. Bulletin of Mineralogy, Petrology and Geochemistry 2: 114-115.
  14. Yin JZ, Chen YC, Zhou JX (1996) Geology and geochemistry of host rocks of the Dashuigou independent tellurium deposit in Sichuan Province, China. Journal of Changchun University of Earth Sciences 26: 322-326.
  15. Yin JZ, Chen YC, Zhou JX (1996) Geology and geochemistry of altered rocks of the Dashuigou independent tellurium deposit in Sichuan Province, China. Journal of Xi’an College of Geology, 18: 19-25.
  16. Chen YC, Yin JZ, Zhou JX (1994) The first and only independent tellurium ore deposit in Dashuigou, Shimian County, Sichuan Province, China. Geol. Sinica 3: 109-113.
  17. Chen YC, Yin JZ, Zhou, JX (1994) Geology of the Dashuigou independent tellurium deposit of Sichuan Province. Acta Geoscientia Sinica 29: 165-167.
  18. Yin JZ, Zhou JX, Yang BC (1994) Rock-forming minerals and ore-forming minerals of the Dashuigou tellurium ore deposit unique in the world – A preliminary study. Geol. Sinica 3: 197-210.
  19. Yin, JZ, Chen YC, Zhou JX (1994k) Mineralogical research of the Dashuigou independent tellurium deposit in Sichuan Province, China. Bulletin of Mineralogy, Petrology and Geochemistry 3: 153-155.
  20. Yin JZ (1996) New mineralogical data of telluride. Bulletin of Mineralogy, Petrology and Geochemistry 15: 246-248.
  21. Liu AP, Zhong ZC, Tang JW (1996) Geochemical characteristics of the Dashuigou tellurium deposit in Sichuan Province of China. Geochemistry 25: 365~371.
  22. Shi HY, Chen YC,Yin JZ, Zhou JX (1996) X-ray diffraction data of telluride. Journal of Mineralogy and Petrology 16: 31-33.
  23. Yin JZ, Shi HY (2020) Mineralogy and stable isotopes of tetradymite from the Dashuigou tellurium deposit, Tibet Plateau, southwest China. Scientific Reports 10.
  24. Yin JZ, Chen YC, Zhou JX (1995) K-Ar isotope evidence for age of the first and only independent tellurium deposit. Chinese Science Bulletin 40: 1933-1934.
  25. Li BH, Cao ZM, Wen CQ (2000) Fluid inclusion type and geological characteristics of the Dashuigou tellurium deposit in Sichuan Province, China. Bulletin of Mineralogy, Petrology and Geochemistry 4: 346-347.
  26. Li TY, Liu JQ (2000) Discussion of analytical methods of grouped fluid inclusion compositions. Geology and Mineral Resources of South China 4: 64-67.
  27. Li BL (1986) Physical chemistry diagram of gas components of fluid inclusions in minerals. Geochemistry 2: 126-137.
  28. Chen PR, Lu JJ, Wang RC (1998) Study on the fluid inclusions of the Dashuigou independent tellurium deposit in Shimian County, Sichuan Province, China. Mineral Deposit 17: 1011-1014.
  29. Li BH, Cao ZM, Jin JF, Wen CQ (1999) Physicochemical conditions of the tellurium deposit in Dashuigou, China. Scientia Geologica Sinica 34: 463-472.
  30. Wang RC, Shen WZ, Xu XJ, Lu JJ, Chen XM, et al. (1995) Isotopic Geology of the Dashuigou tellurium deposit in Sichuan Province, China. Journal of Nanjing University (Natural Sciences) 31: 617-624.
  31. Shen WZ, Xu XJ, Wang RC (1997) Origin of the fluid inclusions of the Dashuigou tellurium deposit in Sichuan Province, China – hydrogen and oxygen isotope evidence. Journal of Nanjing University (Natural Sciences) 33: 77-83.

Studying End of Life Conversations: Challenges and Strategies

DOI: 10.31038/IJNM.2021221

 

End-of-life (EOL) conversations continue to be challenging for patients, families, and healthcare providers (HCPs) [1-3]. Although these dialogues can be emotionally charged, they are critical to ensure that care is aligned with patient preferences. Interventions directed at improving communication about EOL care have been shown to improve patient outcomes. Nurses are in a unique position to assist patients and families with advocating for EOL conversations [4,5]. Advance care planning studies are prominent in EOL literature with the intent of clarifying life sustaining treatment preferences of patients. Unfortunately, most EOL decisions are still made without direct input from patients but rather loved ones are burdened with deciding whether or not to continue life sustaining interventions. In addition, family members report that they were unaware of their loved ones wishes and values with all of the treatment options [6]. More research is needed to develop practical approaches and strategies to enhance EOL conversations to properly align patients’ priorities of care. But conducting these studies remains challenging.

Challenges: First, recruitment can be extremely problematic if the study is targeting patients with specified prognosis. Most HCPs are uncomfortable and lack the knowledge to prognosticate accurately. They are uncomfortable with approaching eligible patients. Additionally, due to variability in patient conditions, availability of patients due to treatments and tests, it is often difficult to schedule interviews. Loved ones are often unavailable during the regular daytime hours. Nurse and HCPs are frequently busy caring for patients. The interviewers are often faculty university members with busy teaching and/or clinical schedules. Other challenges of clinician and stakeholder engagement include the struggle to find convenient, uninterrupted interview times for patients, loved ones and nurses. Furthermore, even if the above challenges are overcome, the interviewers may have discomfort in initiating EOL discussions with both patients and loved ones. Strategies: With the proper study inclusion criteria education and support from more confident, experienced colleagues, nurses can be coached to identify appropriate participants for EOL research. Providing a script to begin the conversations has been shown to be valuable. EOL investigators should expect participant recruitment challenges and plan for ongoing education and support of referral staff. Researchers should plan regularly scheduled debriefing sessions with interviewers to provide emotional support and encouragement to minimize distress. Allocating resources to infuse research into the workplace should involve flexible staffing for participant referral identification and time allocation for interviews of patients and nurses. Providing a scripted approach such as the Patient Preferences About Serious Illness (PASI) [7-9] to introduce the topic may ease HCPs discomfort and allows patients the opportunity to have open, honest dialogues. Ultimately, ongoing discussions between the patient, loved ones and HCPs are the goal throughout the course of the serious illness. More research is needed to improve the process of eliciting EOL discussions between patients and their HCPs and in designing tools such as the PASI. By acknowledging and anticipating the difficulties of having honest EOL dialogues, researchers can tailor strategies to minimize the barriers while promoting opportunities to engage patients, loved ones and HCPs.

References

  1. Trachsel M, Irwin SA, Biller-Andorno N, Hoff P, Riese F (2016) Palliative psychiatry for severe persistent mental illness as a new approach to psychiatry? Definition, scope, benefits, and risks. BMC Psychiatry 16: 1-7.
  2. Scott J, Owen-Smith A, Tonkin-Crine S, Hugh Rayner, Paul Roderick, et al. (2018) Decision-making for people with dementia and advanced kidney disease: A secondary qualitative analysis of interviews from the Conservative Kidney Management Assessment of Practice Patterns Study. BMJ Open [crossref]
  3. Abdul-Razzak A, Heyland DK, Simon J, Ghosh S, Day AG, You JJ (2019) Patient-family agreement on values and preferences for life-sustaining treatment: results of a multicentre observational study. BMJ Support Palliat Care [crossref]
  4. Torke AM, Hickman SE, Hammes B, Steven R Counsell, Lev Inger, et al. (2019) POLST Facilitation in Complex Care Management: A Feasibility Study. Am J Hosp Palliat Med. 36: 5-12. [crossref]
  5. Shaw M, Shaw J, Simon J (2020) Listening to Patients’ Own Goals: A Key to Goals of Care Decisions in Cardiac Care. Can J Cardiol. 36: 1135-1138. [crossref]
  6. Foglia MB, Lowery J, Sharpe VA, Tompkins P, Fox E (2019) A Comprehensive Approach to Eliciting, Documenting, and Honoring Patient Wishes for Care Near the End of Life: The Veterans Health Administration’s Life-Sustaining Treatment Decisions Initiative. Jt Comm J Qual Patient Saf 45: 47-56. [crossref]
  7. Whitehead PB, Clark RC (2016) Addressing the challenges of conducting research with end-of-life populations in the acute care setting. Appl Nurs Res. 30: 12-15. [crossref]
  8. Whitehead PB, Carter KF (2017) A model for meaningful conversation in serious illness and the patient preferences about serious illness instrument. J Hosp Palliat Nurs.
  9. Whitehead PB, Ramalingam N, Carter KF, Katz K, Harden S (2016) Nurse practitioners’ perspectives on the patient preferences about serious illness instrument. J Hosp Palliat Nurs.

Squamous Cell Carcinoma in a Dog (Canis familiaris) Treated by the Viscum album Therapy – Case Report

DOI: 10.31038/IJVB.2021533

Abstract

Squamous Cell Carcinoma is defined as a common malignant neoplasm in the clinical routine of small animals. The treatment of choice includes surgical removal, chemotherapy, radiotherapy, cryosurgery, most of which result in mutilation, deformation, side effects, metastases, and recurrences. Therapy failure is reported by several authors and depends on the disease stage and the patient’s age and health condition. The need for developing new therapies for cancer treatment is well-known, aiming the prophylaxis, treatment, and, when possible, cure of the disease. Under this scope, the Viscum album therapy has been used for more than 100 years by human medicine with reported success in several types and stages of cancer. Therefore, this work describes the administration of ultra-diluted Viscum album in a dog diagnosed with oral SCC. The disease was successfully cured, which was later confirmed by histopathology analysis.

Keywords

Cancer, Viscum album, Dog, Therapy

Introduction

Squamous Cell Carcinoma (SCC) is defined as a relatively common malignant neoplasm, accounting for 5% of all skin tumors. It is considered the second most common oral cancer type (31%) in dogs and cats [1], behind oral melanoma (44%) and prior to oral fibrosarcoma (25%) [2]. However, SCC can arise in various locations in the body [1,3]. In humans, it accounts for 20% of all skin cancers, resulting in 1 million cases in the United States each year. Most cutaneous SCCs are treated locally. However, there is a high incidence of recurrences, metastases, followed by death [3].

The SCC resulting from the oral mucosa epithelium is locally invasive, being considered a severe disease and directly dependent on the stage of the disease’s evolution. It is considered a deforming pathology due to the rapid infiltration of neoplastic cells in the tissues, orofacial destruction, and possible metastases [4].

The SCC etiology is probably multifactorial. Several risk factors are involved, including the use of flea collars and history of industrialized feeding in cats [1,5].

The treatment of choice for oral SCC is surgical, which is considered the best option. However, most of the time, patients are mutilated [5]. Early SCC diagnosis and treatment are essential since initial stage tumors are the most amenable to treatment and with the best prognosis [1]. Surgery, radiotherapy, chemotherapy, and their combinations are also possible options for this treatment. Nevertheless, they have already been tested and rarely presented a satisfactory response. An integrative treatment approach appears to be a better option for successful therapy in these cases [5]. In addition, one must consider animals in advanced stages of the disease and those in palliative care. These patients can benefit from such therapies with regard to improving their quality of life until their death, providing them dignity. In situations like those mentioned above, an approach within integrative therapies would probably offer a better chance of success due to the limitations of the treated patients [5].

Within this context, Valle and Carvalho [6] refer to administering the Viscum album therapy for prophylaxis, treatment and/or cure of cancer patients in its various stages, including animals in palliative care. Viscum album is an excellent treatment option for cancer patients as it has a bidirectional action stimulating the immune system of patients and selectively exerting cytotoxicity against tumor cells [7]. Besides that, it does not have side effects on the body. For this reason, the Viscum album therapy has been used for more than 100 years by human medicine with reported success in several types and stages of cancer [8]. This study aimed to report the case of a 16-year-old female dog with a diagnosis of oral SCC, treated using an ultra-diluted Viscum album.

Case Report

A 16-year-old female Maltese dog weighing 3.9 kg, fed with commercial food, was seen at NaturalPet Clinic in Brasilia, DF, Brazil. The patient was diagnosed with a well-differentiated SCC (60%; Figure 1) four weeks before the veterinary appointment at NaturalPet. The biopsy report carried out by the Histopato Laboratory (Brasilia, DF, Brazil) details that approximately 60% of the analyzed fragment was affected by neoplastic, hypercellular, homogeneous, infiltrated, not well-demarcated, and non-encapsulated lesion. The neoplastic cells were arranged in cohesive blocks bordering concentric and cohesive keratin sheets (keratin pearl). The cells were polygonal with moderate and eosinophilic cytoplasm, rounded nucleus, dispersed chromatin, single and evident nucleolus. Vessels and margins were affected by neoplastic cells.

fig 1

Figure 1: Photomicrograph A. Arrow: Keratin pearl. B. Arrow: Vascular involvement by neoplasm (Photo: Laboratory of Pathology – Histopato, Brasilia-Brazil).

On physical examination, the patient showed aggressive behavior and, at the slightest attempt to touch, she already reacted in a very hostile way. Therefore, the examination was performed very carefully. The animal had normal-colored mucous membranes, CPT 2″, cardiac and respiratory auscultation within the expected range for age and species, hydration conditions within the normal range, moderate sialorrhea accompanied by halitosis. An ulcerated lesion was observed, and the fur was stained with blood around it. The lesion was located below the upper lip, exacerbating gingival mass (Figure 2). The impossibility of manipulating the patient made the examination difficult, but the tutor helped with the procedure. A protocol was instituted using the Viscum album therapy, and it consisted of the first application of Viscum album D3 (1 X 10-3), intravenously, every seven days, for four weeks, associated with the administration of Viscum album D3, subcutaneously, one ampoule (1.1 mL), once a day, on alternate days, initially for 30 days. The subcutaneous applications were performed by the tutor. In addition, it was recommended to replace the commercial diet with a natural diet consisting of a low-carbohydrate recipe (15%). Blood was also collected for laboratory tests of complete blood count and biochemical measurements of alanine aminotransferase (ALT), alkaline phosphatase (AP), urea, and creatinine.

fig 2

Figure 2: Appearance of the initial lesion. Blue arrow: increased volume of the upper lip with edema. Yellow arrow: mass adhered between upper lip and gum, diagnosed by biopsy and histopathology as squamous cell carcinoma.

Results

The results of the blood tests showed – Red blood cells: 7,200,000/uL; Hemoglobin: 15.9 g/dL; Hematocrit: 45%; MCV: 62.5 fL; MCHC: 35.33 g/dL; Leukocytes: 27.400/uL; Platelets: 308.000/uL; ALT – 58 U/L; AP: 61 U/L; Creatinine: 0.61 mg/dL; Urea: 32 mg/dL.

The patient returned to the clinic for the second Viscum album intravenous application, and the mass had surprisingly reduced about 90% of its total size, compared to the initial lesion, remaining only a slight edema on the upper lip (Figure 3). The tutor reported improvement in the animal’s overall condition, improved appetite, sleep quality, and mood. The animal also returned to show interest in playing as she previously used to do. After 30 days of treatment, the affected site was completely restored (Figure 4).

fig 3

Figure 3: Appearance of the lesion after seven days of treatment with Viscum album subcutaneous applications on alternate days. Blue arrow: residual edema in the upper lip.

fig 4

Figure 4: Appearance of the region previously diagnosed with SCC. Result of the treatment 30 days after Viscum album applications. Both images demonstrate the disappearance of the tissue between the upper lip and gum, previously seen, and the edema reduction in the superior lip.

Eight months after the complete reestablishment, the animal returned to the clinic for dental prophylaxis. On this occasion, a new fragment was collected of the region previously affected and diagnosed as SCC. The sample was sent for histopathology analysis to the VetPat Laboratory in Campinas, SP, Brazil. The result showed the presence of fibroconjunctive tissue with the prevalence of scar tissue. Thus, the medication (Viscum album D3) was maintained, but only once a day, three times a week, subcutaneously. However, the animal died (acute renal disease) six months after the dental prophylaxis procedure, at 17 years.

Discussion

Cancer is the primary cause of death or euthanasia in veterinary patients. The oral cavity is in fourth place with the highest incidence, being behind only the mammary gland, genitals, and skin. In general, oral tumors are only noticed when the disease is already in an advanced clinical stage [9,10].

Among oral neoplasms, SCC has been considered a malignant and ulcerative epidermal tumor with a poor prognosis, depending on the stage of the disease evolution [11]. SCC is considered the second most common oral malignant neoplasm in dogs [2] and one of the most commonly diagnosed tumors in humans [12]. It has already been described in the lips, gums, tonsils, oral mucosa, and tongue [13,14]. In many cases, metastasis occurs by lymphatic vessels, mainly to the mandibular region and/or retropharyngeal lymph nodes and lungs [14]. However, in the case here reported, no sign of metastasis was observed in the physical evaluations performed while the animal was monitored until the day of its death. This fact could probably be due to the well-differentiated characteristic of the tumor since metastasis seems to be related to the differentiation degree of neoplastic cells, the most likely occurrence in tumors with little differentiation [15].

The treatment for cancer patients aims primarily at the eradication of the affected tissue, prioritizing the preservation of the functional structure of the affected area, and when possible, its aesthetic appearance [10]. Several options are available for treating this disease in small animals, such as surgical excision, systemic and/or intralesional chemotherapy, radiotherapy, cryosurgery, and immunotherapy [16]. According to Wiggs and Lobprise [17] and Gioso [18], surgery therapy is the most efficient among these options.

However, most conventional treatments do not contemplate the total cure of the disease, nor do they excel in the patient’s life quality, causing, several times, various side effects and the occurrence of metastases. In contrast to the therapies of choice or conventional therapies, this report affirms the effectiveness of treatment here used for treating SCC, which did not have side effects on the patient and resulted in the total cure of the disease, improving the patient’s quality of life, as stated by the tutor.

According to Gioso [18], a favorable prognosis with no or reduced tumor recurrences would demand surgical resection with a wide safety margin. Such procedures may cause significant deformations, mutilations, altering the functional structure of the organ affected. There are also complications associated with the postoperative period of maxillary or mandibular resection, which may include anorexia, tongue projection, difficulty in grasping food, palatal ulcer due to malocclusion, dehiscence of the surgical wound, infection, oronasal fistula, epistaxis, disorders in the salivary gland ducts, and excessive salivation [19]. Contrasting these authors, the prognosis of the present case indicated that depending on the tumor stage the Viscum album therapy should be considered a real option for treating cancer patients, either as a palliative treatment or even aiming at the patient’s cure.

The findings here reported corroborate with Valle and Carvalho [6], who referred to the healing of ulcerated lesions caused by SCC in the breast in a patient in palliative care. However, this study not only showed the resolution of an ulcerated lesion but also demonstrated the complete cure of the process, which was confirmed by an incisional biopsy performed ten months after the initial SCC diagnosis and by the absence of metastasis recurrence.

Conclusion

These data meet the need for new treatment options for cancer patients at different disease stages. The ultra-diluted Viscum album has been shown to be an excellent treatment option for these patients, whether in search of a cure for cancer or just in search of palliative care. Further studies are still needed to confirm the effectiveness of such effects in patients in the various stages of the disease.

Conflict of Interest

The authors declare that there is no conflict of interest in this case report.

References

  1. Webb JL, Burns RE, Brown HM, LeRoy BE, Kosarek CE (2009) Squamous cell carcinoma. Compend Contin Educ Vet 31: 133-142.
  2. Gardner DG (1996) Spontaneous squamous cell carcinomas of the oral region in domestic animals: a review and consideration of their relevance to human research. Oral Dis 2: 148-154. [crossref]
  3. Waldman A, Schmults C (2019) Cutaneous Squamous Cell Carcinoma. Hematol Oncol Clin North Am 33: 1.
  4. Thomson PJ (2018) Perspectives on oral squamous cell carcinoma prevention-proliferation, position, progression and prediction. J Oral Pathol Med 47: 803-807. [crossref]
  5. Bilgic O, Duda L, Sánchez MD, Lewis JR (2015) Feline Oral Squamous Cell Carcinoma: Clinical Manifestations and Literature Review. J Vet Dent 32: 30-40. [crossref]
  6. Valle ACV, Carvalho AC (2021) Homeopathic Viscum Album on the Treatment of Scamous Cell Carcinoma Lesion in a Dog (Canis familiaris) – Case Report. Integr J Vet Biosc 5: 1-3.
  7. Valle ACV, Carvalho AC, Andrade RV (2021) Viscum Album – Literature Review. Int J Sci Res 10: 63-71.
  8. Valle ACV (2020) In vitro and in vivo evaluation of the ultra-diluted Viscum album efficacy and safety. Doctorate dissertation. Catholic University of Brasilia – UCB, Brasilia-DF, Brazil, 78.
  9. Cotran RS, Kumar V, Collins T (2000) Neoplasia. In: Structural and functional pathology. 6th ed. Rio de Janeiro: Guanabara Koogan 233-241.
  10. Howard PE (2002) Maxillary and mandibular neoplasms. In Bichard SJ, Sherding RG (Eds) Saunders Manual – Small animal clinic. 2nd ed. Roca: São Paulo 1181-1189.
  11. Rodaski S, Werner J (2008) Skin neoplasms. In Daleck CR, De Nardi AB, Rodaski S (Eds) Oncology in dogs and cats. Roca: São Paulo 253-279.
  12. Uma RS, Naresh KN, D ́Cruz AK, Mulherkar R, Borges AM (2007) Metastasis of squamous cell carcinoma of the oral tongue is associated with down-regulation of epidermal fatty acid binding protein (E-FABP). Oral Oncol 43: 27-32. [crossref]
  13. Dhaliwal RS, Kitchell BE, Maretta SM (1998) Oral tumors in dogs and cats. Part I. Diagnosis and clinical signs. Compend Continuing Educ Vet 20: 1011-1021.
  14. Meuten DJ (2002) Tumors of the alimentary tract. Tumors of domestic animals. 4th ed. Iowa: Iowa State Press 509-546.
  15. Denis MM, Ehrhart N, Duncan CG, Barnes AB, Ehrhart EJ (2006) Frequency and risk factors associated with lingual lesions in dogs: 1,196 cases (1995-2004). J Am Vet Med Assoc 228: 1533-1537. [crossref]
  16. Mayer MN, Anthony JM (2007) Radiation therapy for oral tumors: Canine acanthomatous ameloblastoma. Can Vet J 48: 99-101. [crossref]
  17. Wiggs RB, Lobprise HB (1997) Veterinary Dentistry, Principles and Practice. Philadelphia: Lippncott-Raven 748.
  18. Gioso MA (2007) Neoplasia da cavidade oral. In: Veterinary odontology for the small animal clinician. 2nd ed. Sao Paulo: Manole 91-100.
  19. Kessler M (2006) Mandibulectomy and maxillectomy as a treatment for bone invasive oral neoplasia in the dog – a retrospective analysis of 31 patients. Europ J Comp Anim Pract 16: 73-82.

COVID Face Masks and the Wuhan Lab Escape Theory: An Update

DOI: 10.31038/JNNC.2021422

Developments Concerning the Wuhan Lab Leak Theory

Only a few months ago, a commentary, with supporting evidence, and a supporting quotation from the former head of the CDC, that the Wuhan lab leak theory for the origin of the COVID-19 epidemic “is a rational, reasonable, and scientifically grounded theory” could have been characterized as a conspiracy theory [1]. Now, a few months later, the Wuhan lab leak theory is being acknowledged widely in the mainstream media as just that – rational, reasonable, and scientifically grounded [2,3]. The fact that Chinese military experiments have been conducted at the Wuhan Institute of Virology has recently been confirmed by Mike Pompeo, a former US Secretary of State and former Director of the CIA [3], and is stated on a US Government website [4]: “Despite the WIV presenting itself as a civilian institution, the United States has determined that the WIV has collaborated on publications and secret projects with China’s military. The WIV has engaged in classified research, including laboratory animal experiments, on behalf of the Chinese military since at least 2017.” https://ge.usembassy.gov/fact-sheet-activity-at-the-wuhan-institute-of-virology/

A few months ago, such a statement would have been dismissed as a conspiracy theory. The mainstream media is now pointing out that gain of function research on coronaviruses at the Wuhan Institute of Virology was funded by the NIH and NIAID [2,3]. In 2015, researchers from the United States and the Wuhan Institute of Virology published a paper in a medical journal stating that gain of function research at the Wuhan Institute of Virology was funded by the NIH and NIAID [5]. Several quotations from this paper confirm this fact: “In addition to offering preparation against future emerging viruses, this approach must be considered in the context of the US government–mandated pause on gain-of-function (GOF) studies. (p. 1512) On the basis of these findings, scientific review panels may deem similar studies building chimeric viruses based on circulating strains too risky to pursue, as increased pathogenicity in mammalian models cannot be excluded. (p 1512) In developing policies moving forward, it is important to consider the value of the data generated by these studies and whether these types of chimeric virus studies warrant further investigation versus the inherent risks involved. (p. 1513)”

The paper describes the contributions to this gain of function research by coauthor Xing-Ye Ge at the Wuhan Institute of Virology. Despite this paper being published in 2015, until recently anyone claiming that the NIH and NIAID funded gain of function research on coronaviruses at the Wuhan Institute of Virology would have been dismissed as a conspiracy nut, and likely as a racist xenophobe. In a February, 2021 article [6], Dr Peter Ben Embarek, speaking as a member of the WHO investigation team on the origins of the COVID-19 pandemic, was quoted as dismissing the Wuhan lab leak theory. Dr. Embarek is the President of Eco Health Alliance which acted as a conduit for the NIH and NIAID funding of coronavirus gain of function research at the Wuhan Institute of Virology [2]. Funding of this research by Eco Health Alliance is acknowledged by the authors of the 2015 paper reporting that research [7].

Former US Secretary of State and Director of the CIA, Mike Pompeo was quoted as saying in a May 25, 2021 [8] article that: “It was outrageous to see scientists, even government, U.S. government scientists who were denying this when they surely must have seen the same information that I had seen,” Pompeo said. “That includes certainly Dr. Fauci as well.” https://www.foxnews.com/politics/pompeo-outrageous-fauci-lab-leak-theory. Dr. Fauci has been denying that NIAID funded gain of function research at the Wuhan Institute of Virology. He and Dr. Francis Collins, Director of the NIH both denied this in recent testimony to the US House Appropriations Committee. A video of Dr. Fauci making this denial is available along with a May 25, 2021 article on the topic [5]. There are only two possible explanations for Dr. Fauci’s denial of NIH and NIAID funding of coronavirus gain of function research as the Wuhan Institute of Virology: 1) he is not telling the truth, or 2) he has no idea what is going on at NIAID. Gain of function research means modifying viruses to make them more infectious and more deadly. Need we ask why the Chinese military has been funding research at the same lab in the same time period? The United States government has funded such research at the Wuhan Institute of Virology, so the US should not be pointing a finger at China.

It is an important question whether the COVID-19 pandemic originated due to the virus jumping from bats to humans in the wild or from a leak at the Wuhan Institute of Virology. However, it is more important to investigate the disinformation originating from medical leadership in the United States and the discrediting of anyone who questions government public health policies concerning COVID-19 – the discrediting is done with charges of being anti-scientific, a conspiracy nut, a racist, and a xenophobe. Curiously, but not surprisingly, the charge of anti-science is often brought against ‘conspiracy nuts’ by people who themselves are anti-scientific.

Things are changing, however. A May 28, 2021 article [9] discusses a paper in press at Quarterly Reviews of Biophysics Discovery [10] that provides compelling evidence that the COVID-19 virus was engineered at the Wuhan Institute of Virology as part of gain of function research conducted there. According to the author of the May 28 article, this research by Sorensen, Susrud and Dalgleish, who have previously published in the same journal [11], was rejected by a number of journals before finally being published. The May 28 article states that: [Sorensen, Susrud and Dalgleish] said they tried to publish their findings but were rejected by major scientific journals which were at the time resolute that the virus jumped naturally from bats or other animals to humans.

Even when former MI6 chief Sir Richard Dearlove spoke out publicly saying the scientists’ theory should be investigated, the idea was dismissed as ‘fake news.’ Over a year later, leading academics, politicians and the media finally flipped, and have begun to contemplate the possibility that COVID-19 escaped from the Wuhan Institute of Virology in China – a lab where experiments included manipulating viruses to increase their infectiousness in order to study their potential effects on humans. Soon, hopefully, physicians and researchers will no longer be blackballed for taking the Wuhan lab leak theory seriously.

Licensing Body Sanctions against Physicians Who Question Public Health Policies about Face Masks

We are now moving into a phase of the COVID-19 pandemic in which centralized government and regulatory bodies are threatening physicians with sanctions if they question public health policies concerning face masks for COVID-19 protection. The College of Physicians and Surgeons of Ontario recently issued a Statement concerning COVID-19 [12]: The College is aware and concerned about the increase of misinformation circulating on social media and other platforms regarding physicians who are publicly contradicting public health orders and recommendations. Physicians hold a unique position of trust with the public and have a professional responsibility to not communicate anti-vaccine, anti-masking, anti-distancing and anti-lockdown statements and/or promoting unsupported, unproven treatments for COVID-19. Physicians must not make comments or provide advice that encourages the public to act contrary to public health orders and recommendations. Physicians who put the public at risk may face an investigation by the CPSO and disciplinary action, when warranted. When offering opinions, physicians must be guided by the law, regulatory standards, and the code of ethics and professional conduct. The information shared must not be misleading or deceptive and must be supported by available evidence and science.”

It is a scientifically proven fact that face masks have no effect on the transmission of viruses in public and no effect on rates of disease [13-16]. This fact is based on multiple randomized controlled trials and meta-analyses. Any Ontario physician who tells patients this scientific fact is now in danger of being censored and sanctioned by the Ontario College of Physicians and Surgeons of Ontario. Perhaps, in another year, leading physicians will be stating that mandating of face masks was a well-intentioned social control tactic but is not supported by science. Perhaps medical authorities will be citing non-existent ‘newly emerging data’ as a justification for no longer requiring face masks in public, even for unvaccinated persons. Or perhaps not. Perhaps we will be stuck with a pandemic of iatrogenic anxiety based on fears about no longer wearing face masks.

A concluding question: what is the difference between a conspiracy theory and the truth? Answer: 6-12 months.

References

  1. Ross CA (2021) Misinformation concerning face masks and the Wuhan lab leak. Journal of Neurology Neurocritical Care 4: 1-3.
  2. Knapton A (2021) Why the Covid Wuhan lab escape theory, dead and buried months ago, has risen again. https://www.yahoo.com/news/why-covid-wuhan-lab-escape-152301731.html.
  3. Shaw A (2021) Pompeo says Wuhan lab was engages in military activity alongside civilian research. https://www.yahoo.com/news/pompeo-says-wuhan-lab-engaged-154413535.html.
  4. https://ge.usembassy.gov/fact-sheet-activity-at-the-wuhan-institute-of-virology/
  5. Chamberlain S (2021) Fauci admits ‘modest’ NIH funding of Wuhan lab but denies ‘gain of function’. https://nypost.com/2021/05/25/fauci-admits-nih-funding-of-wuhan-lab-denies-gain-of-function/.
  6. Newey S (2021) Expert from WHO origins investigation warns against relying -too much on US intel’ on COVID-19. https://www.telegraph.co.uk/global-health/science-and-disease/expert-origins-investigation-warns-against-relying-much-us-intel/
  7. Menachery VD, Yount BL Jr, Debbink K, Agnihothram S, Gralinski LE, et al. (2015) A SARS-like cluster of circulating bat coronaviruses shows potential for human emergence. Nature Medicine 21: 1508-1513. [crossref]
  8. Olson T (2021) Pompeo says it’s ‘outrageous’ US officials, including Fauci, dismissed lab leak theory. https://www.foxnews.com/politics/pompeo-outrageous-fauci-lab-leak-theory.
  9. Boswell J (2021) Covid-19 ‘has NO credible natural ancestor’ and WAS created by Chinese scientists who then tried to cover their tracks with ‘retro-engineering’ to make it seem like it naturally arose from bats, explosive new study claims. https://www.dailymail.co.uk/news/article-9629563/Chinese-scientists-created-COVID-19-lab-tried-cover-tracks-new-study-claims.html.
  10. Sorensen B, Susrud A, Dalgleish AG (in press) A reconstructed aetiology of the SARS-Coronavirus-2 spike. Quarterly Reviews of Biophysics Discovery.
  11. Sorensen B, Susrud A, Dalgleish AG (2020) Biovacc-19: A candidate vaccine for Covid-19 (SARS-CoV-2) developed from analysis of its general method of action for infectivity. Quarterly Reviews of Biophysics Discovery. doi: 10.1017/qrd.2020.8.
  12. College of Physicians and Surgeons of Ontario. https://www.cpso.on.ca/News/Key-Updates/Key-Updates/COVID-misinformation.
  13. Ross CA (2020) Differences in evaluation of hydroxychloroquine and face masks for SARS-CoV-2. Journal of Neurology and Neurocritical Care 3: 1-3.
  14. Ross CA (2020) Thoughts on COVID-19. Journal of Neurology and Neurocritical Care 3: 1-3.
  15. Ross CA (2020) Facemasks are not effective for preventing transmission of the coronavirus. Journal of Neurology and Neurocritical Care 3: 1-2.
  16. Ross CA (2020) How misinformation that facemasks are effective for reducing COVID-19 is transmitted. Journal of Neurology Neurocritical Care 3: 1-2.

Treatment of Fibropapillomatosis in a Green Sea Turtle (Chelonia mydas) Using Ultra-Diluted: Case Report

DOI: 10.31038/IJVB.2021532

Abstract

Fibropapillomatosis (FP) is an infectious, neoplastic disease, frequently found in sea turtles, whose prevalence may be associated with environmental factors and predisposition, generally associated to herpesviruses presence, compromising, their locomotor, ocular and food apprehension performance. In the present report, the presentation of fibropapillomas occurred in the ocular region. The diagnosis is based on physical examination, histological and PCR, while conventional treatment involves surgical removal of tumors, cryosurgery and electrochemotherapy, accompanied by recurrences. However, at present, we chose homeopathic treatment with Thuya occidentalis 30 cH, in which it was effective and less harmful to the patient, since there were no recurrent conditions.

Keywords

Fibropapiloma, Homeopathy, HPV, Thuya

Introduction

Fibropapillomatosis (FP) is a neoplastic infectious debilitating disease, commonly found in sea turtles, presenting multiple, large and ulcerated skin masses, characterizing the presence of fibropapilloma and internal fibroma that compromise locomotion and food apprehension [1]. The origin can be developed as a result of environmental factors and genetic predisposition, usually caused by a herpesvirus [2,3] with latency capacity in the host organism [4]. Thus, being the prevalence of the disease associated with unfavorable environmental conditions, it is possible through it, monitor the state of ecosystems [5].

FP is also considered a panzootic and emerging disease, threatening the conservation of the species, in addition to causing serious locomotor damage, in vision, in the food apprehension and increasing the predisposition to secondary diseases [2,6,7]. Conventional treatment for FP involves surgical removal of tumors, cryosurgery and electrochemotherapy, subject to complications [8,9], while the cure of homeopathic treatment promoted by the administration of Thuya occidentalis, is lasting effectiveness and also less harmful [10].

Case Report

One green turtle (Chelonia mydas), juvenile, female, with free life, found on the Itaipuaçú beach, in Maricá in Rio de Janeiro State (Figure 1A), presenting fibropapillomatosis in several areas of it body and in the right cornea, being submitted to 4 surgical procedures to remove the fibropapillomas, because its always presented recurrence and evolution in the size of the fibropapilloma of the right eye region.

fig 1

Figure 1A: 04/28/2018

Considering the enucleation of the left eye of the sea turtle and impaired vision of the right eye, it was not possible to reintroduce it into nature, being forwarded to Sabina – Escola Parque do Conhecimento, located in Santo André/SP (Figure 1B).

fig 2

Figure 1B: 01/23/2019

Given the above, we opted for homeopathic treatment with Thuya occidentalis 30 cH on 01/23/2019, whose treatment evolution was monitored weekly through biometrics and photographic record of fibropapilloma.

In March 2019, it was observed that fibropapilloma remained stable, but without size evolution, as observed in the previous conventional treatment. The administration of HPV Biotherapic 30 cH was added to the protocol, which after 15 days, there was regression of the size of the fibropapilloma (Figure 2). In the month of July 2019 it was entitled to increase the potency of the HPV biotherapic to 200 cH and decrease its frequency once a week until March 2020, attesting to complete regression without recurrence (Figure 3).

fig 3

Figure 2: 03/27/2019

fig 4

Figure 3: 04/17/2020

Discussion

The origin of fibropapillomatosis is infectious as a result of environmental factors, such as climate change, environmental degradation and especially the human disturbances in marine environments [11] and genetic predisposition, that the transmission is caused by a herpesvirus, as evidenced in research carried out by Tamar Project with FMVZ – USP [2]. The study confirmed the presence of Chelonid Alphaherpesvirus 5 (ChHV5) associated with fibropapillomatosis in three types of tissue: skin, tumor and blood sample [12]. Research shows that in Florida and Caribbean, this disease is expanding, but in the other hand, decreasing in Hawaii [13]. This fact can be associated with the divergence of nucleotides between populations [14]. The most memorable feature of the virus is it latency capacity in the host organism, remaining in the infected animal’s organism for long periods, entering the active phase of the infection, in moments of homeostatic discontinuity [4,15].

Tumors are more frequently located in the soft tissue of the turtles’ skin, at the base of the tail, close to the eyes and in the oral, cervical, inguinal, axillary and hull regions, causing impairment in the functioning of the purposes, vision and increased susceptibility diseases of associated eye structures, food apprehension, breathing and efficiency in the escape of predators [2,6]. In the present report, the presentation of fibropapillomas occurred in several areas of the body, including the ocular region. The frequency in the cranial region, including the ocular region, was only 1.01% of the cases, while the most frequent area is the fins region (47.05%) [7]. Corroborating, a study only 4 of the 787 animals (0.5%) were found with corneal fibropapillomas with varieties sizes, similar thickness and microscopic characteristics, different as reported in others studies (United States and Hawaii) [6].

That it may have a relationship of environmental change, showing that the prevalence of the disease is associated with water quality, pollution of coastal areas, areas with high human density, large contribution of agricultural, domestic, industrial and marine biotoxins, in such a way that the etiology of fibropapillomatosis can also serve to monitor the health of ecosystems [10,5,16]. A study with organic pollutant, polycyclic aromatic hydrocarbons, showed that turtles with FP had higher concentrations of this pollutant in the liver, than those that did not have FP [16]. Affected animals may also present impaired status, fluctuation disorders, cachexia, hypoproteinemia, uremia and elevation of liver enzymes, while in hematological indices, non-regenerative anemia, progressive decrease in lymphocyte count, basophils and eosinophils and progressive increase in heterophiles and monocytes [2].

Comparatively, the patient presented anemia in the first exam, which it obtained normalization in the new exam after 1 year, as well as an improvement in leukopenia from 8 thousand/mm³ to 11.6 thousand/mm³; normalized lymphopenia from 9% to 20%; and ALT that wes increased by 35 U/L, decreasing to 25 U/L.Macroscopically the tumor lesions varying from smooth to verruciform with small pointed projections, pigmented of white, pink, red, grayish, purple or black color, also varying in size and conditions of ulceration and necrosis [1,2]. In the case of the present report, the lesions were whitish with a rough appearance.

Conventional treatment recommends surgical removal of tumors with a common scalpel blade, electric scalpel, cryosurgery or carbon dioxide laser [2,17]. However, one study found that 38.5% of post-operative green turtles patients, has the tumors returned after 36 days [3] as well as the sea turtle in this study. The complications of surgical removal include difficulty in surgical closure of large areas of tissue, risk of secondary infection or anemia, anesthetic risk and a high rate of recurrence [9]. Even because of the slow metabolism of the species, the healing time of sea turtles can be extremely long, representing open surgical wounds, potential portals of entry for secondary infections, which can lead to the animal’s death [8].

The electrochemotherapy has been used for the treatment of epithelial neoplasms in several animal species in an efficient and safe way to treat fibropapillomas in turtles, being free from adverse effects or without tumor recurrence, however the investment of this treatment is high [8]. In this case, homeopathic treatment was chosen in order to promote an efficient and less harmful treatment based on the Law of Similarity was adopted, aiming at the balance of the vital force of the patient, which the individual’s own organism fights the aggressor agent [18].

Therefore, the protocol adopted was with Thuya occidentalis 30 cH, due to its proven action in reducing inflammatory responses in animals, involving reduction of edema, inhibiting the migration of neutrophils at the site of inflammation, reducing vascular permeability and pro-inflammatory cytokines, oxidative stress, in addition to an immunomodulatory effect [10]. In order to increase the speed of remission of fibropapillomas, the patient received HPV Biotherapic 30 cH as a association, maintaining the homeostasis of the organism, in an ideal process where the cure occurs through the reestablishment lasting health. In a study made with Magellanic penguins, pododermatitis was treatedquicklu and effectively with homeopathy [18]. Due to the slow metabolism of the reptiles, the treatment occurred for a longer period to observe the remission of the condition [3], exactly the opposite of birds that respond more quickly to homeopathy due to their faster metabolism [18,19].

Conclusion

Ultra-diluted drugs have shown their effectiveness on the total reduction of fibropapiloma without causing recurrence, commonly observed in conventional treatment. So the animal’s greater well-being is valued, without subjecting it to surgical and anesthetic procedures.

Acknowlegments

We thank all members of Sabina – Escola Parque do Conhecimento.

Conflicts of Interest

The authors declare that they have no conflicts of interest.

References

  1. Rossi S, Sanchez-Sarmiento Am, Vanstreels Ret, Dos Santos Rg, Prioste Fes, et al. (2016) Challenges in evaluating the severity of fibropapillomatosis: a proposal for objective index and score system for green sea turtles (Chelonia mydas) in Brazil. PLoS ONE 11: 1-11. [crossref]
  2. Matushima ER, Longatto Filho A, Di Loretto C, Kanamura CT, Sinhorini IL, et al. (2001) Cutaneous papillomas of green turtles: a morphological, ultra-structural and immunohistochemical study in Brazilian specimens. Braz J Vet Res Anim Sci 38: 51-54.
  3. Page-Karjian A, Serrano ME, Cartzendafner J, Morgan A, Ritchie BW, et al. (2020) Molecular assessment of chelonid alphaherpesvirus 5 infection in tumor-free green (Chelonia mydas) and loggerhead (Caretta caretta) sea turtles in North Carolina, USA, 2015-2019. Animals 10: 1964. [crossref]
  4. Marschang RE (2011) Viruses Infecting Reptiles. Viruses 3: 2087-2126. [crossref]
  5. Jones K, Ariel E, Burgess G, Read M (2016) A review of fibropapillomatosis in Green turtles (Chelonia mydas). Vet J 212: 48-57. [crossref]
  6. Flint M, Limpus CJ, Patterson-kane JC, Murray, PJ, et al. (2010) Corneal fibropapillomatosis in green sea turtles (Chelonia mydas) in Australia. J Comp Path 142: 341-346. [crossref]
  7. Silva Junior ED (2016) Incidência de fibropapilomatose em tartarugas marinhas na bacia Potiguar RN/CE, Pós Graduação em Biologia Estrutural e Funcional para titulo de mestre Universidade Federal do Rio Grande do Norte – UFRN. 2016.
  8. Brunner CHM, Dutra G, Silva CB, Silveira LMG, Martins MFM (2014) Electrochemotherapy for the treatment of fibropapillomas in Chelonia mydas. J Zoo Wildl Med 45: 213-218. [crossref]
  9. Donnelly KA, Papich MG, Zirkelbach B, Norton T, Szivek A, et al. (2019) Plasma bleomycin concentrations during electrochemotherapeutic treatment of fibropapillomas in green turtles Chelonia mydas. J Aquat Anim Health 31: 186-192. [crossref]
  10. Remya VK, Kuttan G (2014) Homeopathy remedies with antineoplastic properties have immunomodulatory effects in experimental animals. Homeopathy 1-9. [crossref]
  11. Yetsko K, Farrell JA, Blackburn NB, Whitmore L, Stammnitz MR, et al. (2021) Molecular characterization of a marine turtle tumor epizootic, profiling external, internal and postsurgical regrowth tumors. Commun Biol 4: 1-16. [crossref]
  12. Lawrance MF, Mansfield KL, Sutton E, SAVAGE AE (2018) Molecular evolution of fibropapilloma-associated herpesviruses infecting juvenile green and loggerhead sea turtles. Virology 521: 190-197.
  13. Work TM, Dagenais J, Willimann A, Balazs G, Mansfield K (2020) Differences in antibody responses against Chelonid Alphaherpesvirus 5 (ChVH5) suggest differences in virus biology in ChHV5-Seropositive green turtles from Hawaii and ChHV5-Seropostive green turtles from Florida. J Virol 94: 1-15. [crossref]
  14. Morrison CL, Iwanowicz L, Work TM, Fahsbender E, Breitbart M, et al. (2018) Genomic evolution, recombination, and inter-strain diversity of chelonid alphaherpesvirus 5 from Florida and Hawaii green sea turtles with fibropapillomatosis. Peer J 2: 1-33. [crossref]
  15. Cárdenas DM, Cucalón RV, Medina-Magües LG, Jones K, Alemán RA, et al. (2019) Fibropapillomatosis in a Green Sea Turtle (Chelonia mydas) from the Southeastern Pacific. J Wildl Dis 55: 169-173. [crossref]
  16. Vilca FZ, Rossi S, De Olinda RA, Sánchez-Sarmiento AM, Prioste FES, et al. (2018) Concentrations of polycyclic aromatic hydrocarbons in liver samples of juvenile green sea turtles from Brazil: Can these compounds play a role in the development of fibropapillomatosis? Mar Poll Bull 130: 215-222. [crossref]
  17. Mader DR, Wyneken J, Weber ES, Merigo C (2006) Medical Care of Sea Turtles: Medicine and Surgery. In: Reptile Medicine and Surgery 76: 977-1000.
  18. Narita FB, Scardoeli B, Gallo Neto H, Coelho CP (2020) Homeopathic treatment of pododermatitis in magellanic penguins (Spheniscus magellanicus). Homeopathy. [crossref]
  19. Coelho C, Von Ancken ACB (2019) Uso da homeopatia na medicina clínica e preventiva de animais selvagens. Boletim técnico ABRAVAS Brasil.

Review on Divalent Atom and Group in Bioisosterism

DOI: 10.31038/JPPR.2021421

Abstract

Bioisosterism in rational drug design approach specifically the divalent atom and group in their modification and optimization to improve the pharmacokinetic and pharmacodynamics properties of compound have been studied.

Keywords

Bioisosterism, Divalent

Introduction

Bioisosterism is a strategy of Medicinal Chemistry for the rational design of new drugs, applied with a lead compound (LC) as a special process of molecular modification [1]. The LC should be of a completely well-known chemical structure and possess an equally well-known mechanism of action, if possible at the level of topographic interaction with the receptor, including knowledge of all of its pharmacophoric group. Furthermore, the pathways of metabolic inactivation [2], as well as the main determining structural factors of the physicochemical properties which regulate the bioavailability, and its side effects, whether directly or not, should be known so as to allow for a broad prediction.

There are two kinds of divalent isosteres. They are:

One that involves the swapping of an atom that is involved in the double bond such as C=C, C=N, C=O, and C=S.

The other types are those when substituting atoms of different kinds result in the alteration of a single bond. such as seen here:

C-C-C, C-NH-C, C-O-C and C-S-C.

In the structural activity relationship study of many different active pharmacological agents, both types of isosteres substitution have been used widely.

Divalent Replacement Involving Double Bonds

As mention above this group involves such replacements as C=C, C=N, C=O, and C=S. tautomerization of these groups is facilitated by the presence of a heterocyclic system in the lead compounds while studying therapeutic agents and the absence of moving proton migrating in the ring system. Replacing C=S with C=O in the aldose reductase inhibitor Tolerstat, which is being studied for its activity in diabetic neuropathy had produced oxo-Tolerstat which have both in- Vivo and in-vitro [3].

{[6-methoxy

{[6-methoxy 5(trifluoromethyl) naphthalene-1-carbothioyl] (methyl) amino} acetic acid.

Aldose reductase inhibitory activity of Tolrestat and Oxo- Tolrestat.

inhibition table

Inhibition of enzyme activity in partially purified bovine lens preparation. 2, Inhibition of galactitol accumulation in the sciatic nerves of rats fed 20% galactose for 4 days. This class of divalent isosteres can also be used with purine nucleoside analogs that are tested for in- Vivo antiviral activity against the Semliki Forest virus [4]. The percentage of mice surviving for 3 weeks for different bioisosteric analogs compared to the absence of control group mice survivors. A weakly active compound has resulted when a sulfur replacement at C-8 is done with oxygen or selenium.
3rd

2-amino-9-[3,4-dihydroxy-5-(hydroxymethyl) oxolan-2-yl]-1,9-dihydro-6H-purin-6-one (Table 1).

Table 1: Guanosine bioisosteric analogues activity against semliki Forest Virus.

S.no

C-8 %Total survivor
1 C=S

83 (10/12)

2

C=O 67 (8/12)
3 C=Se

58 (7/12)

Divalent Replacement Involving Single Bonds

These Bioisosterism are a group of an atom which are attached to different substituent attachment of two different substituents to the Bioisosterism the polar and chemical difference is less noticeable. The biological activity of these divalent bioisosteres hold could be linked to the bond angle. In the example below the relationship between the anti-allergic activity and bond angle of different divalent bioisosteres is shown [5].
Aminophylline

Aminophylline orally at 100 mg/kg was used as a positive control and assigned a biological response of (++); (-) not significantly different from negative control group at p<0.05 as determined by the Dunnett’s t test; (+) activity between positive and negative groups; (++) activity equivalent to positive control group; (+++) activity greater than positive control group. Passive foot anaphylaxis is an IgE mediated model used to detect compounds that have anti-allergic action. . Using oxygen as a bioisosteric linker shows a smaller bond angle and greater electronegativity proving an improved potency. Another good study on inhibitors of nuclear factors of activated T-cell (NFAT) – mediated transcription of β- galactosidase. T-cells are the main component of the immune system that gets activated on the contact with foreign matter induction interleukin-2 (IL-2) gene which are necessary autocrine growth factor for T-cells. Activated T-cell release many different bioactive molecules to initiate a series of events that leads to immune /inflammatory response [6]. The region 257- 286 base pairs upstream of the IL-2 structural gene binds to a protein, the nuclear factor of activated T cells-1 (NFAT-1), preceding to IL-2 gene transcription. NFAT-1 is conveyed in relatively few cells besides T cells and is markedly upregulated upon stimulation of the T cell receptor. This makes it an extremely specific target within activated T cells. When the cell is activated, the NFAT-1 protein binds to the DNA at its recognition site and induces the transcription of â-galactosidase. This study evaluated some of the bioisosteric analogs of quinazolinediones as an immunosuppressant agent since they can inhibit β- galactosidase (Tables 2 and 3).
last

Table 2: Oral anti-allergic activity in the passive foot anaphylaxis’s Assay of analogous containing varied heteroatom.

S.no

x Electronegativity Bond angle Passive foot anaphylaxis’s (10 mg/kg)
1 -O- 3.15 108.0

+++

2

-S- 2.32 112.0 +
3 -CH2- 2.27 111.5

+

4

-NH- 2.61 111.0

+

Table 3: Regulation of NFAT-1- regulated β-galactosidase activity by Quinazolinediones is shown below.

S.no

X IC50 (µM)
1 -NH-

4.47

2

-CH2 4.03
3 -O-

2.5

Conclusion

Bioisosteres control biological activity by virtue of restrained differences in their physicochemical properties. Systematic correlation of physicochemical parameters with observed biological activity has been very effective in highlighting subtle differences within bioisosteric groups which frequently increase activity. Of significance is the ability of these bioisosteric groups to describe some of the essential requirements of the pharmacophore. This is especially important when the synthesis of a large number of drug nominees for assessment is not economically achievable. A number of less known replacements have not been reviewed because of their inability to demonstrate bioisosterism in more than a single class of agents. In this review, an attempt has been made to explain the rationale behind the use of bioisosteric replacements using examples in divalent atom and group from current literature. It is hoped that this systematic approach will facilitate the use of bioisosteric replacements in future structure activity studies.

Author Contribution

The manuscript was written through contributions by the author Hawi Matewos Daka.

Acknowledgment

Finally, I would like to thank my parents Matewos Daka and Bayush Temesgen for the emotional and financial support they have given me to write the article.

References

  1. Burger AA (1983) Guide to the Chemical Basis of Drug Design. NY, EUA Wiley.
  2. Stenlake JB (1979) Fondations of Molecular Pharmacology, Vol 2. The Chemical Basis of Drug Action, Londres, Inglaterra, Athlone Press pp: 213-290.
  3. Wrobel J, Millen J, Sredy J, Dietrich A, Kelly JM, et al. (1989) Orally Active Aldolase Reductase Inhibitors Derived from Bioisosteric Substitutions on Tolrestat. J Med Chem 32: 2493-2500. [crossref]
  4. Bonnet PA, Robins RK (1993) Modulation of Leukocyte Genetic Expression by Novel Purine Nucleoside Analogues. A New Approach to Antitumor and Antiviral Agents. J Med Chem 36: 635-653. [crossref]
  5. Walsh DA, Franzyshen SK, Yanni JM (1989) Synthesis and Antiallergy Activity of 4-(Diarylhydroxymethyl)-1-[3-(aryloxy)propyl] piperidines and Structurally Related Compounds. J Med Chem 32: 105-118. [crossref]
  6. Michne WF, Schroeder, JD, Guiles JW, Treasurywala AM, Weigelt CA, et al. (1995) Novel Inhibitors of the Nuclear Factor of Activated T Cells (NFAT)-Mediated Transcription of â-Galactosidase: Potential Immunosuppressive and Antiinflammatory Agents. J Med Chem 38: 2557-2569. [crossref]

Field Study on Abortion Storm in Dromedary Camel Farm in Saudi Arabia with Emphasis to Chlamydiosis

DOI: 10.31038/IJVB.2021531

Abstract

In a semi – intensive farm system for Camelus Dromedarius, native Saudi camel breeds were kept for milk purpose in north area of KSA. Mijaheem subspecies was dominant in number due to the believe that they are the highest in milk production. In December 2018 sudden continuous abortions were encountered without any apparent causes. Abortions were in the last trimester and were very high compared to previous years’ records. Samples were collected and sent to laboratories locally and aboard, ELISA and PCR real – time were conducted accordingly and farm field measures were implemented intensively. The study summarizes the whole investigation been conducted and the results been stated with clear evidence of Chlamydia abortus as a cause of the abortion storm.

Keywords

Chlamydia, PCR real time, north of Saudi Arabia, Camelus dromedarius, Abortion

Introduction

Abortion refers to pregnancies that terminate with the expulsion of fetus with recognizable size prior to the period of viability, which is arbitrarily defined as 260 days for cow and 290 days for mare. Also fetal death is not an essential prelude to abortion. Abortion may be spontaneous or induced, infectious or noninfectious [1]. As for Camelus dromedaries Saeed Basmael found that 389 days are the maximum number of days that camel fetus can stay viable inside the uterus of his dam [2]. The authors have applied this information to declare abortion in camelus dromedarius is the expulsion of fetus of recognizable size prior to 389 days of gestation.

Chlamydia has a worldwide distribution causing a wide range of disease in human hosts, livestock, companion animals and even wild and exotic species. Chlamydiosis in animals can range from asymptotic infection to severe disease with life-threatening illness [3] Chlamydial taxonomy lately settled to describe order Chlamydiales consisting of nine families, first famous family is Chamydiaceae. This family Chlamydiaceae consist of one single genus Chlamydia which include 11 (Eleven) species: these species are C. abortus; C. caviae; C. felis; C. muridarum; C. suis; C. pecorum; C. avium; C. psittaci; C. pneumonia; C. trachomatis; C. gallinacea [4]. Virtually chlamydial species might easily cross all body host barriers. Members of chlamydiales order are obligate intracellar Gram negative bacteria which are transmitted as metabolically inactive and must differentiate, replicate and re-differentiate within the host cell to carry out their life cycle. It is obvious that due to science development Chamydiales order life cycle is being greatly understood [5].

In Saudi Arabia the first record of serological evidence of camel chlamydiosis was reported by Mansour F. Hussein, ELISA test was performed by Chlamydophila abortus enzyme immunoassay kits (IDEXX LAB. USA). 19.4% of the tested camel were positive for anti-chlamydial antibodies which was more prevalent in females than males and also higher in adult than young camels [6]. Abdelmalik Kalafalla was the first to use PCR real-time technique for detection of chlamydiosis in indigenous camels of Saudi Arabia using uterine swabs and reported 10.3% positive for chlamydiosis [7]. Also I Al Khalifa reported chlamydiosis in 10.05% samples from 378 Mijaheem camels been tested using indirect enzyme-linked immunosorbent assays [8]. These above 03 chlamydiosis prevalence reports in Saudi Arabia camels are great evidence for scientists to continue research in this field especially with the high rate of abortions encountered in Saudi Arabia now. There are also reports of camel chlamydiosis in nearby countries such as in UAE [9]; Egypt [10]; Tunisia [11]; Libya by [12] and in Algeria by [13]. Moreover, scientists declared that ticks can carry viable chlamydophila and can transmit it to other animals [14,15]. Zoonotic infections due to chlamydia abortus and chlamydia psittaci are reported and well known and more prevalent than other Chlamydia species [3]. Vertical transmission, which promotes the persistence of infection in ruminant herds, also occurs in birds, venereal transmission of C. abortus is possible since the bacteria are found in the semen of bulls, rams and goats [16].

Materials and Methods

Farm Management System

The location case farm is highly organized and have a very large space area which reach 30000 Hecter, in far north of Saudi Arabia. There is a well-built management system with ear tags electronic identification (SHEARWELL Co.) and the camel have been kept in an isolated area of more than 6000 Hecter which give them good area to practice natural grazing and also as natural barrier against contacts with other camel herds around, so eliminating different diseases contacts possibilities. The farm has a simple model of camel milking parlor and also a milk processing plant is located 15 KGM from camel zone serving camel and goat milk. The rearing management system is mainly divided into two major units:

a. Unit one: Mating, grazing and calving

b. Unit two: Milking

Unit One: Mating, Grazing and Calving

After mating, primitive tail rising method of pregnancy diagnosis is practiced and positive ones ate separated into specific and been fed an according to the farm policy waiting for a second pregnancy check. Mating and tail checking is going on during the winter season which is between October up to April of next year and also the separation process of positive pregnant and rechecking are continuing on. In the end of the winter season all the group of she camels are drove out to the grazing unit (mainly to decrease the feed cost). Negatives are kept alone for more investigations. In the camel area of the project also there are many central water pivots which belong to the company agricultural dept. and are always grown barley, Alfa-Alfa, corn or industrial tomato so a good chance of grazing in these pivots after harvesting are well utilized and of high benefit. A daily check of animal health and feeding are done perfectly and all necessary programmed and interventions are practiced. The well clear pregnant ones are drove back to calving pens on approaching the coming season and calving and calf management is done, then they transferred to milking unit and the cycle is repeated.

Unit 2: Milking

On receive of dams with their calves after spending 15 days in calving pens and been naturally direct udder feeding been practiced, all dams with their calves will start a training programmed of machine milking procedures. After some time, the dam will be trained and will come alone to the parlor without her calf. Usually dams are milked twice a day and kept for one milking season, but sometimes it will continue milking for up to 18 months. Animal health and feeding depts. are available according to the procedures.

Case Report

In 2018 and while the calving season was going smoothly as previous seasons (season usually between Oct. and April of next year), calving attendants report a series of abortions in the last trimester stage of pregnancy without any reason or prior symptoms, they only had found aborted foeti and may found some links to a certain dam due to drooled placenta or some fluids and blood on the outer dam gentilia. More procedures were implemented and night supervisors appointed with instruction to call veterinarian to attend all deliveries or abortions any time night or noon. Three management approaches were implemented which are:

a. Effective quarantine of the calving area was done, closing of the camel area also implemented and separate labors for the calving area was strictly observed and controlled. We build a new calving area for new comers from grazing area to deliver calves.

b. Disinfections were applied to pens, feeders, drinkers and the camels in daily basis. Vitamins and minerals mixers were applied to camels feed and water as recommended by manufacturers. Mass antibiotic water soluble treatment was practiced for all adult females whether aborted or not with Doxycycline-200 W.S. powder at a dose of 05 Grams per 200KG body weight for 05 consecutive days in drinking water. The treatment programmed continued monthly for 03 days with the same drug and dose till end of parturition season.

c. Importantly samples from recent aborted attended cases were collected, preserved and been sent to local and aboard laboratory. We had taken from 20 aborted she camels blood for serum for ELISA testing in ARASCO Co. in KSA (Table 1). From other 08 aborted she camels, we had collected amniotic fluids, blood for serum, part of placenta tissue and a preputial wash from one male camel and been sent to aboard laboratory (Table 1).

Table 1: Samples details

Samples and specimen

Quantity

Laboratory and intended test
1. Blood for serum

20

KSA ARSCO Co. for ELISA test
2. Amniotic fluid, blood for serum, placenta tissue and preputial wash

08

Aboard laboratory for PCR

Results

Closely we trace the abortion rate of the concerned location from the records of year 2014 up to year 2018 season in which sudden sharp rising abortion incidents were noted and recorded. Also we have the data of 2019-year season which the abortion rate come back to normal like rates of previous years before the abortion storm of year 2018. In Table 2 below we can check easily the rates of the abortion in the location from year2014 up to end of year 2019 calving season.

From Table 2 it clearly indicated how it was serious and difficult situation to encountered suddenly a continuous silent abortion in year 2018 which finally reached 33.80% of the total pregnant she camels. But lucky enough in year 2019 the abortions came back normal after a tremendous effort were excreted to deal with the storm outbreak of abortions.

Table 2: Abortions % from year 2014 to year 2019

Year

Total pregnant she

Total calves alive Total aborted calves

Abortion rate

2014

204

199 05

02.45%

2015

246

244 02

00.81%

2016

132

132 00

00.00%

2017

142

142 00

00.00%

2018

210

139 71

33.80%

2019

152

146 06

03.95%

ELISA Results

20 frozen serum samples were prepared and been sent to ARASCO Co. which usually do testing (customer services) in King Saud University at faculty of animal and food science (Table 3).

Table 3: ELISA results

Disease Antibodies tested

Total samples

Positive samples

Toxoplasma

20

20

Q – Fever

20

16

Chlamydia

20

04

Brucella spp

20

08

Johne’s disease

20

14

The ELISA technique was performed according to manufacturer’s procedure using CHEKIT enzyme immunoassay kits from IDEXX Laboratories Inc., USA.

PCR – Real Time Results

The following samples have been collected from 07 attended aborted she camels, which were blood sera, amniotic fluids and placenta tissue. One male preputial wash also was collected and all samples been frozen and sent aboard to a known laboratory and the following results were being received from them (Table 4).

Table 4: PCR – real time results

Disease tested

Total samples

Positive samples

Toxoplasma gondii

08

00

Coxiella burnetti

08

00

Chlamydia abortus

08

06 (05 female+01 male*)

Brucella spp

08

00

Mycobacterium ovium

08

00

*The male revealed positive in PCR and was removed from the herd permanently.

The samples for PCR real-time were prepared in cooled ice box and immediately been sent via road to Jordan University of Science and Technology, veterinary laboratory in faculty of veterinary science, (Jordan is only about 170 KMs from the farm). Results were posted via Email to the farm management in Dec 19,2018. As in Table 4, 6 samples were positive for C. abortus and all samples were negative to T. gondi, Cox burnetti, Br. Abortus and M. ovium.

Discussion

Low reproduction performance in camels is mainly ascribed to old age at first calf. Long calving intervals and limited breeding season [17]. Camels birthing rates rarely exceed 40% in nomadic herds and 70% in semi or intensive herds and neonatal mortality is huge and may reach epizootic proportions [18]. Tibary also stated that abortions in Camelus dromedarius due to infectious diseases vary from 10% to 70% and Brucellosis and Trypanosomiasis represent the major causes of infectious abortions in the Middle East and Africa (Tibary 2016). In Saudi Arabia the first record of serological evidence of camel chlamydiosis was reported by Mansour F. Hussein [6].

In this study an unexpected storm of abortions in pregnant female camels in the third trimester period of gestation occurred without any previous signs of illness neither before abortion, nor after it. As it is clear from the Table 2 it was the first time to report an outbreak of abortions (33.80%) of the total pregnant females that year aborted. The location is an intensive farming system of dromedary camels designed for milk production and have a commercial automatic milking parlor and a dairy plant. From farm records abortion rates were never exceeded 02.45% for the last five years (2014-2018). PCR – real time have detected positive cases of females and one male infected with Chlamydia abortus. ELISA results of the incident indicate presence of antibodies for five infectious diseases namely Toxoplasma, Q. Fever, Brucella spp., Johne’s disease and Chlamydia which agree with Abdelmalik study who was the first to use PCR real-time assays for detection of uterine infections indigenous camels of Saudi Arabia. Chlamydiosis, toxoplasmosis and Brucellosis were detected in this study and Abdelmalik [7].

More investigations are needed especially when there is a clear abnormal rate of abortions or reproductive failures. This study should direct the attention of owners, farm veterinarians and research centers to the importance of Chlamydia as one of serious emerging causes of abortions in Camelus dromedarius. The Elisa results indicate the presence of antibodies against Toxoplasma, Q-fever, Brucella and Johne’s disease so more care should be alert for more investigations and further studies. The intensive management care and treatments been practiced in the farm had led to stop abortions at a rate of 33.80%. In second reproduction season (2019-2020) the abortion rate was only 3.95%. From the field attendance, abortion nature, data collected and laboratory results the authors have great confidence it is a Chlamydiosis outbreak incidence and should be a corner stone in any future investigations of Camelus dromedarius abortion cases.

Conclusion

Chlamydiosis should be considered as a major emerging cause of abortions in Camelus dromedarius in Saudi Arabia, Middle East and Africa due to increased studies done recently and have proved its importance as a cause of abortions and reproductive failures in camel production. Prevention of infectious causes of reproductive losses and abortions in camelids should be based on sound biosecurity measures designed to prevent the introduction and spread of disease in population, herd or group of camelid in specific area. These measures can simplify in vaccination programs of specific diseases, pre-breeding reproductive examinations like uterine culture and cytology, males also must be tested physically and microbiologically, quarantine of recently introduced camels, breeding hygiene should be strictly observed, pre-parturient monitoring and personnel attending parturient females should be practiced to recognize any abnormalities.

References

  1. HAFEZ ESE (1980) Reproduction in Farm Animal. Fourth Edition.
  2. Saeed Basmael (1996) Modern Breeding of camel Dairy population (In Arabic) Publication of agriculture extension centre Riyadh/Saudi Arabia.
  3. Nicole Borel, Adam Polkinghorne, AndreasnPospischil (2018) A review on Chlamydial Disease in Animals: Still a challenge for pathologists. Veterinary Pathology 55: 374-390. [crossref]
  4. Sachse K, Bavoil PM, Kaltenbocck B, Stephens RS, Kuo CC, et al. (2015) Emendation of the family Chlamyiaceae: proposal of a single genus, Clamydiato include all currently recognized species. Syst Appl Microbiol 38: 99-103. [crossref]
  5. Yasser M, Abdel Rahman, Robert J Belland (2005) The chlamydial development cycle. FEMS Microbiology Reviews 29: 949-959.
  6. Mansour F, Hussein M. ALShaikh MO, Gad El EL-Rab, ALjumaah RS, et al. (2008) Journal of Aninaml and Veterinary Advances 7: 685-688.
  7. Abdelmalik I Kalafalla, Marzook M Al Eknah, Mahmoud Abdelaziz, Ibrahim M Ghoneim (2017) A study on some reproductive disorders in dromedary camel herds in Saudi Arabia with special references to uterine infections and abortion. Trop Anim Health Prod 49: 967-974. [crossref]
  8. Khalifa IAL, ALshaikh MA, ALjumaah RS, Jarelnabi A, Mansour F Hussein (2018) Journal of Animal Research 8: 335-343.
  9. Wernery U, Wernery R (1990) Seroepidemiology studies of the detection of antibodies to Brucella, Chlamydia, Leptospira, BVD/ MD virus, IBR LIBV virus and enzootic bovine leucosis virus (EBL) in dromedary mares (camelus dromdarius). Deutsche Tierarztliche Wochenschrift 97: 134-135. [crossref]
  10. Schmatz HD, Kraus H, Viertel P, Ismail, Hussein A (1978) Acta Tropica 35:101-111.
  11. Burgmeister R, Leyk W, Gossler R (1975) Cited by Hussein et al., 2008.
  12. Elzlitne R, Elhafi G (2016) Seroprevalence of Chlamydia abortus in camel in the western region of Libya. J Adv Vet Animal Res 3: 178-183.
  13. Mohamed Hocine Benaissa, Nora Mimoune, Curtis R Youngs, Rachid Kaidi, Bernard Faye (2020) First report of Chlamydophila abortus infection in dromedary camel (Camelus dromedaries) population in eastern Algeria. Comparative Immunology, Microbiology and Infectious Diseases 73:101557.
  14. Croxatto A, Rieille N, Kernif T, Bitam I, Abeby S, et al. (2014) Presence of Chlamydiales DNA in ticks and fleas suggests that ticks are carrier of Chlamydiae. Ticks Ticks Dis 5: 359-365. [crossref]
  15. Burnard D, Weaver H, Gillett A, Loader J, Flanagan C, et al. (2017) Novel Chlamydiales genotypes identified in ticks from Australian wildlife. Parasit Vectors 10: 46.
  16. Rodolakis A, Mohamad KY (2010) Zoonotic potential of Chlamydophila. Vet Microbiol 140: 382-391.
  17. Eiwishy AB (1987) Reproduction in the female dromedary (Camelus dromedaries): A review. Animal Reproduction Science 15: 273-297.
  18. Tibary A, Fite C, Anouassi A, Sghiri A (2006) Infectious causes of reproductive loss in camelids. Theriogenology 66: 633-647. [crossref]

Social and Business Problems through the Lens of Projective Iconics: Introducing a New Systematics to Understand and Quantify Perceptions of Social Issues

DOI: 10.31038/PSYJ.2021323

Abstract

We introduce a new approach to understanding the mind of people regarding the solution of social issues, an approach we title Projective Iconics. The objective is to understand the ‘mind’ of the respondent regarding the solution of a problem, using a projective technique incorporating Mind Genomics. Respondents were presented with a social problem: securing affordable access to medical help. The objective was to understand how they responded to the problem, based upon their reactions to test vignettes, combinations of names of 16 individuals. The names represented different positions of authority, personality, and behaviors. The vignettes were constructed by combining names to create a group of individuals tasked with the job of solving the issue. Each respondent rated 24 unique vignettes. Experiment 1 instructed the respondent to evaluate the likelihood that the group of individuals in a test combination could cooperate to discover a solution. The experimental design enabled the discovery between success and each of the 16 individuals, suggesting three clear mind-sets of respondents, respectively; those who believed that everyday individuals would solve the problem, those who believed that people in power would solve the problem, and those who believed that celebrity personages would solve the problem. When the dependent variable in Experiment 1 was ‘cooperate’, the segmentation into mind-sets was not as clear. Respondents in Experiment 2 estimated the likely total taxes needed to solve the problem, again select the expected taxes that the group in the vignette would levy. Three clusters emerged for responses using taxes as the dependent variables, but the clusters or mind-sets again were not as clear.

Introduction

The study of social problems is often done by what might be called ‘outside in.’ That is, the researcher wants to understand how the person undergoing the problem or situation feels, and constructs a questionnaire, or some other tool to observe behavior. The questionnaire poses a blockade between the researcher and the respondent. The researcher attempts to communicate the nature of the problem, whereas the respondent attempts both to understand the question, and to answer in the appropriate manner. The appropriate answer may be either a truly honest answer in the opinion of the respondent, or perhaps all too often an answer that is that which the researcher might wish to hear. These are so-called respondent or interview biases, better known colloquially as being ‘pc’, viz., politically correct [1-4].

The biases in such interviews are well known, causing the glibly offered remark that ‘one cannot believe what respondent say for many topics where emotion enters.’ In our increasingly polarized political and social environment biases have emerged in polling, so much so that there are difficulties in accurately stating issues without perhaps irritating some respondents. A world of emerging biases and problems might well imperil the development of social science and knowledge about the everyday, simply because people are becoming increasingly sensitized, due to the Internet in general, and social media in particular. A further factor is the ubiquitous survey, whether that be a long market research survey taking 20 minutes, or the irritating pop-up service for customer satisfaction about the transaction just completed. It seems that virtually every transaction seems to be followed by a request for information, and a subtle desire to be uprated.

With the advent of computer technology it is becoming increasingly easier to track a person’s behavior, especially on the Internet, but also where the person happens to be in terms of geography. Our cell phone has ‘location’ transponder, allowing both the offeror of services to know where we are for transactions, but also to know and to record where we are for further study, such geographical mapping of people who frequent certain stores. Such improvement in the science of measuring ‘behavior’ has also led to a different thinking. Rather than asking the respondent to give us opinions, measuring and summarizing the respondent’s behavior. It is no wonder that once we purchase something, we receive an unending set of advertisements on our cell phone to buy the same product, at specific store, located very close to where the person happens to be.

One major problem with both questionnaires and with behavioral tracking can be traced to the reality that the information is obtained by an outsider, who can be perceived to be invading a person’s private domain. When the issue is questionnaire or personal interaction, the respondent, may put up conscious or unconscious defenses, perhaps providing incorrect information. When the issue is behavior tracking, what a person does gives little information to what the person thinks in general about the topic, and certainly gives even less information about nuances of thought and feeling.

The Emerging Science of Mind Genomics May Address the Bias Problem

Mind Genomics refers to an emerging psychological science, founded on previous contributions in experimental psychology [5], mathematical psychology [6], personality psychology, specifically projective techniques such as the Rorschach Test [7], and finally using the metaphor of the MRI used in medicine [8].

The goal of Mind Genomics is to measure the values of internal ideas, internal thoughts, in a rigorous way, appropriate to the topic. The foundation of Mind Genomics comes from psychophysics the study of the relation between physical stimuli and subjective percepts. S.S. Stevens, the founding father of modern-day psychophysics suggested having the respondent act as a measuring instrument, to scale the perceived or subjective magnitude of a stimulus. These scaling methods reveal repeatable patterns describing the relation between the stimulus magnitude measured by instruments, and the subjective intensity measured by the person. Such information, generating what is called ‘Outer Psychophysics.’ In the same spirit, albeit with slightly different tools, Mind Genomics attempts to establish the parallel information, the relation between the inner idea and a subjective magnitude. This goal, called ‘Inner Psychophysics’, can be considered to the be the ‘UR story’, the foundation story for Mind Genomics. Psychophysics forms the foundation of measurement, but the story does not stop there.

The typical approach in science, psychophysics included, focus on isolating one factor or variable, occasionally two or three, and even sometimes four variables, changing the variable in a systematic way, measuring the response, and then describing the pattern. The pattern may be a change in the nature of the type of response (qualitative), or the magnitude of the response (quantitative) or both. In psychology, especially in the study of thinking and how we process the information of the everyday, the isolation of a single variable and measuring the response to systematically changed levels of that single variable is popular, but becomes problematic in the study of everyday life, i.e., in the study of the typical situations in which people find themselves. The everyday decisions, those of the ordinary life, involve the interaction of several variables. Studying one variable at time may be fine for artificial laboratory situations but is not fine for the study of common decision-making, for example voting for a political candidate, or buying a product, respectively. The research to study everyday must involve the study of combinations of variables, such combinations created according to an underlying set of procedures called an ‘experimental design’ [9]. In mathematical psychology and in subsequent applications by marketers the focus on one variable at a time has evolved to the focus on several variables simultaneously interacting [6,10]. The approach is called conjoint measurement. It is conjoint measurement which constitutes the second leg of the emerging science of Mind Genomics.

The third ‘leg’ of the Mind Genomics science can be found in the world of projective techniques [11]. The experimental design used by Mind Genomics allows the researcher to present many combinations of independent variables to the respondent, who is instructed to treat the combination as a single idea, a single proposition, and rate that entire proposition on a scale. For the study reported here, one group of respondents evaluated combinations of messages, specifically names of individuals or groups, on the likelihood that the whole group described in the test stimulus could solve a particular social problem, defined as ‘access to medical care.’ The other group were shown the same combinations of messages but instructed to rate the incremental or decremental amount of TAXES that the group would impose on the population to solve the problem.

The final, fourth leg of the research was the creation of a general picture of the mind of the consumer, done by putting together all the combinations, and creating a single equation or model showing how each of the elements drives either the likelihood of solving the problem ((Experiment #1) or how much of an increase in taxes each element would incur to solve the problem (Experiment #2I). The metaphor for this fourth leg is the MRI, magnetic resonance imaging, which takes many pictures of the underlying stimulus, pictures from different angles, and then recombines them afterwards, using a computer program to assemble them into one three-dimensional image [8].

The Two Mind Genomics Studies – Problem/Solution and Taxes

The studies reported here were occasioned by the discussions among the authors on different occasions about the need to systematize social science research, and if possible, bring to it the rigors of experimentation such as those found in experimental psychology, and especially in psychophysics. The notion was to create an integrated database, with the researcher empowered to investigate a range of ‘topics’, here ‘social issues,’ with the same tools, in a manner that might be called ‘industrial-scale research.’ Most of the research to which the authors had been introduced to, and had practiced, required meticulous attention to detail, and were studies which were complete in and of themselves, with very tenuous connections to other data collected by researchers on the same topic. The authors were aware of review papers, which attempted to pull together the diverse and divergent research efforts over many years, and by so doing create a structure by which to better understand the area. These are called review papers or the meta-analyses.

Mind Genomics provides an entirely different approach to the problem, an approach which lends itself to scalability in terms of application to many different problems, generating common data, and inspiring the research to create a ‘data warehouse.’ The governing vision in this study was to apply the Mind Genomics paradigm, explained below, to understand aspects of access to medical care, from the mind of the citizen consumer. As will be explained below, the same approach could be, and indeed was, applied to investigated 26 other social and economic problems. The current paper is just the first of a set of 27 integrated pairs of studies. of the same type.

The Mind Genomics Paradigm Explicated Using the Data Front the Two Studies

Mind Genomics studies follow a simple paradigm, making the research almost programmatic, so-called ‘cookie-cutter’ by those who feel that the acquisition of knowledge in research cannot or should be done in an industrial fashion, viz., scaled-up, rapid, efficient, and inexpensive. Although there are many researchers who frown upon ‘cookie-cutter’ research, feeling that each study must be unique as well as elegant, the value of a standardized, templated, quickly executed method should not be underestimated. The steps below, applied and executed less than 24 hours, from start to finish, provides the researcher with a rare opportunity to create a useful database which addresses many existing questions, opening new vistas by revealing hitherto unexpected patterns in the way people can be shown to ‘think’ about a topic.

Step 1 – Identify the Topic

This step identifies the problem. The actual task is harder than it may sound. We are not accustomed to thinking in terms of tight, limited scopes. The topic here is improving access to medical care at an affordable price.

The study reported here comes from a set of 27 studies on problems, all run in the same way. Table 1 shows the list of the problems, and the language used for each. The structure of analysis for this study shows what can be learned from virtually a superficial plunge into the data analysis, viz., the results which lie at the surface.

Table 1: The topics or problems originally comprising the set of issues to be investigated using this one study.

table 1

Step 2: Create Four Questions Which Tell a Story, and Four Answers to Each Question

The study here on access to medical care does not lend itself easily to the question-and-answer format. Rather, the strategy is to identify four groups of authorities, these authorities being of different kinds. Each authority takes the place of a question. Each of the four specific individuals or groups takes the place of an answer. Table 2 shows the four general groups of authorities, and the four specifics for each authority.

Table 2: The raw material, comprising the four types of authorities, and the four specifics within each type.

table 2

The use of common symbols, viz., people, was done to explore the potential of moving beyond the typical research approach which often use factual descriptive phrases as elements or answers to the questions. Instead, the objective here was to use ‘cognitively-rich’ stimuli, without explanation, allowing the respondent to link these stimuli with the question. The approach here took as its origin the work relating color to feeling, and to the psychophysical method of ‘cross-modality matching,’ where the respondents adjust the perceived intensity of one continuum (e.g., the loudness of sounds) to match the perceived intensity of another continuum (e.g., the brightness of lights). That breakthrough in psychophysics, first reported in the early 1950’s, almost 70 years ago, stimulated the conjecture that perhaps one could match problems to people in a similar way [5].

Step 3: Create a Set of Combinations Using the Principles of Experimental Design

Step 3 creates combinations of the four types of elements (A1-A4, B1-B4, C1-C4, D1-D4). Experimental design was used to create 200 different sets of test vignettes. Each set of test vignettes conformed to the same experimental design, but the elements in the design were permuted, keeping the basic structure of the design, albeit with different combinations [9,12].

Each vignette comprised a specific combination of 2-4 answers or elements, with at most one answer element from a question, but often with no answers. The vignettes were thus not all complete, although the 24 systematically varied combinations comprised a complete experimental design. Each element of the 16 appeared exactly five times in the 24 combinations and was absent 19 times in the 24 combinations. The combinations were set up so that the 16 elements were statistically independent of each other.

These set-up efforts permit the researcher to analyze the data from one respondent as easily as analyze the data from 100 respondents, since at the most granular level each respondent’s data matrix can be analyzed by itself, using standard methods, such as OLS (ordinary least-squares) regression. It will be that property of each respondent’s data following its own complete experimental design which will allow the researcher to create individual-level models, and cluster or group the respondents based upon the pattern of their coefficients. The computer requires the format shown in Table 3 to apply the method of OLS (ordinary least squares) to deconstruct the ratings (or the transformed ratings, see below) into the contributions of each element.

Table 3: Example of three vignettes, combinations of elements, and their recoding into 16 independent variables. For the recoding 1=present, 0 = absent.

Combination A1 A2 A3 A4 B1 B2 B3 B4 C1 C2 C3 C4 D1 D2 D3 D3 D4
1 A1  B4 C4 D4 1 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 1
2 A1 B3 D1 1 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0
3 A4 B4 C1 D4 0 0 0 1 0 0 0 1 1 0 0 0 0 0 0 0 1

Table 3 shows an example of three of the 24 combinations that a respondent will evaluate. The respondent sees actual combinations rather than the combinations. It is at this point that the Mind Genomics paradigm diverges from the more conventional approaches, by assigning each respondent to a different design. This approach differs dramatically from the typical methods in science, which focus on averaging out variability by evaluating the same limited set of stimuli with many respondents, until the mean becomes stable. The Mind Genomics worldview is more like that of the MRI, magnetic resonance imagery. Each experimental design becomes a snapshot. At the end of the study, the modeling combines these snapshots to produce a coherent whole incorporating all the different ‘views’ of the same underlying object being investigated. In our study that ‘object’ is the way people react.

Step 4: Create a Short Introduction to the Topic and Provide a Rating Scale

The introduction should present as little information as possible. Instead of formulating the entire situation in the introduction, the researcher should let the elements themselves, the different groups and individuals to provide the necessary information on which the respondent will assign a judgment. Table 4 shows the orientations for the two experiments, the first study dealing with the ability to cooperate and solve the problem, the second with the expected taxes. Table 4 also shows the rating scale for each study. The rating scale is how the respondent communicates her or his feelings about what has been read. The first experiment allows for five possible responses. These will be subsequent deconstructed to yield four scales, only one of which will be of interest here, the ability to solve the problem. The second experiment, focusing on taxes, uses a more traditional scale, dealing with the expected increase in taxes. There are five options here as well. The options are not in simple order, but rather presented in irregular order, forcing the respondents to give some thought to the issue. Respondents are prevented from simply using the scale as one of magnitude, where the five points are equally spaced, and in order.

Table 4: The two questions and the rating scales.

What will happen when these people work together to solve this problem: Improving access of everyone to good medical care without paying an unaffordable price
1=Cannot cooperate … and … No real solution will emerge
2=Cannot cooperate … but … Real solution will emerge
3=Honestly cannot tell
4=Can cooperate … but … No real solution will emerge
5=Can cooperate … and … Real solution will emerge
What will happen to our FEDERAL TAXES when these people work together to solve this problem: Improving access of everyone to good medical care without paying an unaffordable price
1= 19% increase in Federal Tax
2= 0% increase in Federal Tax
3= 27% increase in Federal Tax
4= 7% increase in Federal Tax
5=11% increase in Federal Tax

Step 5: Follow the Templated Process

Create combinations of vignettes according to an underlying experimental design, present these combinations to the respondents, and obtain both a rating on the appropriate scale, and record response time. Response time is the time between the appearance of the vignette and the assignment of the response by the respondent on the 1-5 scale. The template makes it possible for the researcher to set up a study with 20-30 minutes, launch the study, and have the data back with 30-60 minutes, with the results analyzed.

The important things to keep in mind while doing the experiment revolve around the shift in thinking from confirming one’s hypotheses (the hypothetico-deductive approach) to creating what might be best called a ‘cartography of the mind.’ There need not be any formal hypothesis One is simply measuring responses to variations of stimuli, to identify which variations, which features, drive the responses.

Step 6: Transform the Data

The two rating scales provide information, but the ratings must first be transformed to allow for subsequent analysis by modeling and clustering.

Scale #1, for attitude (cooperate and solve the problem) can be transformed in at least two ways. One way is to create a binary scale for solving the problem. In that case, ratings 1, 2 and 3 are converted to 0 to denote that the problem cannot be solved, whereas ratings 4 and 5 are converted to 0 to denote that the problem can be solved. A second way is to create a binary scale for cooperation. In that case the ratings are converted in a different fashion. Ratings 2 and 5 are converted to 100 to denote that the groups in the vignette can cooperate, whereas ratings 1, 3 and 4 are converted to 0 to denote that the groups in the vignette cannot cooperate to solve the problem.

Our focus in this analysis is on the ability to solve the problem, so that the first transformation is followed, with ratings 1, 2 and 3 transformed to 0, and ratings 4 and 5, in turn, transformed to 100. A small random number, < 10-4, is added to each rating, after transformation. The small random number does not affect the analysis but ensures necessary variation in the dependent variable in the situation where a respondent assigns all vignettes ratings which all end up either 0 or 100, respectively.

Rating scale #2, for tax, is transformed to the relative tax values, in percent. Thus no increase in tax would be transformed to 100, to denote 100% of current taxes. A 27% increase in tax would be transformed to 127, to denote 127% of current taxes.

The transformations provide two types of information. The first is a no (0) or a yes (100), appropriate for the ratings of the first of the two experiments. There is no sense of magnitude, just of no/yes. The second is a magnitude of the effect.

We can get a sense of the basic interest in the data by comparing averages across respondents. Figure 1 (top) shows the distribution of beliefs across the different vignettes that the access problem can be solved. Each filled circle corresponds to one of the 102 respondents. The figure is not particularly interesting. What will be more interesting will be the linkage of the solution to the individuals. In contrast, Figure 1 (bottom), far more interesting, gives a sense of the average impact on the tax expected across a variety of different individuals who would get involved in the effort to provide access. Figures of the type shown here are of basic interest because they deal with a simple quantity, taxes.

fig 1

Figure 1: Distributions of average transformed ratings. The top panel shows the averages across 102 respondents for the belief that the group can solve the problem of affordable access to medical care. The bottom panel shows the average taxes (vs current) to achieve the goal of affordable access to medical care.

Step 7: Build Equations Using OLS (Ordinary Least Squares Regression), without an Additive Constant

At both a group level, and at a respondent level, relate the presence/absence of the 16 elements to the transformed rating of able to solve the problem (study #1), or expected increase in taxes (study #2). For these studies, a single form of the OLS regression was used, one without an additive constant. The rationale for using the equation in without the additive constant is from the desire to compare coefficients across studies (solve the problem vs taxes), and to compare coefficients across transformations (solve the problem versus cooperate).

It is important to note that the pattern of coefficients is similar (high correlation) when one estimates the coefficients using an equation which has the additive constant, versus an equation which is absent the additive constant. The same patterns emerge but the magnitudes of the coefficients differ, being large for the equations lacking the additive constant.

Experiment #1 – Cooperate/Solve (ratings 4 & 5 transformed to 100): Binary Rating = k1A1 + k2A2 + k3A3…K16D4

Each coefficient shows the incremental (or decremental) proportion of responses driving the binary rating. Thus, a coefficient of +15 means that when the element is included in the vignette, 15% more of the responses are 4 or 5. High coefficients suggest strong drivers of the solution; low coefficients suggest weak drivers of the solution.

Experiment #2 – Taxes: Percent of Current Taxes = k1A1 + k2A2 + k3A3 …. K16D4

Each coefficient shows the incremental (or decremental) percent of taxes to be expected when the specific element (individual) is included in the group.

When the analysis is done at the group level, all respondents incorporated into the model, the coefficients are relatively low. For models or equations computed without the additive constant, coefficients around 16 or higher are considered ‘statistically significant’. Comparable elements in models estimated with an additive constant require a value of +8, or higher, just about half.

Table 5 suggests ranges coefficients, but few reaching statistical significance. More important, there are no clear patterns. The patterns will emerge from segmenting the respondents. For response time, only one element generates a long inspection time, A4, the mayor of my city. It is also clear that, at least for the total panel, response time does not covary with the coefficients of the elements.

Table 5: Coefficients from the experiments. The columns correspond to the dependent variables.

table 5

Table 5 also shows response times, based upon using the above models to relate the presence/absence of the 16 elements to the response time. The response time was measured along with the respondent’s rating, allowing OLS regression (without the additive constant) to relate the presence/absence of the elements to the response time. Table 5 no clear relation between coefficients for either cooperate or solve, and coefficients for response time in seconds, although the response time for older respondents was longer than the response time for younger respondents, confirming previous findings for response time versus age [13,14].

Step 8: Cluster the Respondents into a Limited Set of Groups Whose Patterns of Coefficients within a Group are Like Each Other

Clustering is a well-accepted procedure in statistics. Our 16 coefficients for each respondent give us a sense of how the respondent feels about either the ability of the individual/group to solve the problem (experiment #1), or the expected change versus current in the taxes one will incur to solve the problem (experiment #2).

The clustering method used here is called k-means clustering [15]. Clustering puts objects into a limited set of groups based upon the statistical criteria set up at the start of the study. The criteria here was to minimize the ‘distances’ within a cluster, and to maximize the distances between the centroids of the clusters, these being the centroids or average coefficient values of the 16 elements. The measure of distance, D, is defined as the quantity (1-Pearson Correlation Coefficient). The quantity D has the lowest value of 0 when the correlation coefficient is 1 (1-1 = 0), and the highest value of 2 when the correlation coefficient is -1, viz. the two patterns are exactly opposite (1- – 1 = 0).

The clustering was done three times:

a. Cluster the respondents on the basis of pattern of the 16 coefficients estimated for “solve the problem” (Experiment #1).

b. Cluster the respondents using the pattern of the 16 coefficients estimated for “cooperate together” (Experiment #1).

c. Cluster the respondents using the pattern of expected taxes for the 16 individuals (Experiment #2).

Step 9: For each Clustering, Extract Three Mind-sets Based on the Pattern of Coefficients

Tables 6 and 7 show the sorted coefficients from Experiment 1, attitudes. Only positive coefficients are shown, with strong performers shown in green. The cut-point for a strong performing element is a coefficient of 16 or higher, corresponding to the coefficient of +8 or higher for those models or equations estimated with an additive coefficient.

Table 6: Coefficients of the 16 elements, based upon clustering ‘who will solve the problem’ (ratings 2 and 5 transformed to 100).

table 6

Table 7: Coefficients of the 16 elements, based upon clustering ‘who will be able to collaborate to solve the problem’ (ratings 4 and 5 transformed to 100).

table 7

The clustering reveals three clearly different mind-sets emerging from clustering based on the perceived ability to solve a problem. In contrast, the three mind-sets created from the estimated ability to cooperate. The clarity of mind-sets for solving problems contrasts with the rather noisy mind-sets emerging from cooperation.

Table 8 (top) shows the expected percent of taxes attributed to each of the elements, sorted by the highest to the lowest increases. Table 8 (bottom) shows the same data, the same data, this time sorted from bottom up. The mind-sets moderately clear, but not as well defined as the mind-sets emerging from clustering ability to solve problems, but far clearer than the mind-sets emerging from clustering cooperation.

Table 8: Expected percent of taxes to be levied by each of the individuals, responding to the need to provide affordable medical care to the population

table 8

The preparation of the tables was done with the taxes themselves, assigned to each vignette, with the tax replacing the rating number. This means that one can add up the coefficients for the taxes to estimate the relative tax to be levied to solve the problem. As an example, consider mind-set C1. These respondents feel that three groups involved, C3 (Nancy Pelosi), B3 (Working with a high-ranking official from the military – e.g., Chief of Staff), and with A pastor of a very large church – e.g., Joel Osteen, would incur a relative tax of 41% +41% + 39% or 121% of current taxes.

Consider now the response of mind-set C2 to these same three individuals. The relative taxes would be 36 + 30 + 28 or 94%, viz., 94% of current taxes, a 6% tax reduction!

Finally, consider now the respondent of mind-set C3 to these same three individuals. The relative taxes would be 22% + 30% + 34% viz, 86% of current taxes, a 14% tax reduction!

Discussion

The typical approach to social problems involves questionnaires, which allow the respondent to think about the issue in a rational way. To a great degree these suffer from biases of expectation, and political correctness, where the respondent provides an answer consistent with a predetermined from of reference, or an answer that feels intuitively ‘acceptable’. People are sensitive to interviewers, and often want to know the ‘right answer’ even when the interview or survey is conducted on the web, in total privacy. The desire to get the right answer, to outguess and perhaps outfox the researcher, muddies the waters. The respondent may not be able to state at a conscious level that she or he was trying to ‘outguess’ the interwar, but such behavior is far more common than one thinks. In such cases strict controls in design and execution must be taken.

To address the issue of expectations, the development of Mind Genomics began with the presentation to the respondent what the words of Harvard’s noted psychologist, Wm James, might call a ‘blooming, buzzing confusion.’ The combinations seem to be haphazard, but they are not. Furthermore, in study after study the data appears to be meaningful and consistent, making a great deal of sense, and in the words of the research community, ‘telling a coherent story.’ The approach of using these combinations of messages.

This paper moves one step beyond the traditional Mind Genomics studies. Rather than providing simple statements of fact, the study uses names of people. The names themselves carry rich meanings to the individuals. The respondents are not asked to decide based upon intellectual factors. Rather, the respondents are asked to give their ‘gut feel’ based upon the feeling of a set of names with complex meaning. The consequence of the approach is a new way of looking at people and thinking. The objective is to move beyond conscious, purpose-driven evaluations of single ideas, and instead move towards the complexities of everyday life, where decisions are made. Only time will tell whether this incorporation of psychophysics, experimental design, personality psychology, and consumer research methods can live up to the potential of becoming a new way to measure the minds of people for topics that can be considered important parts of ordinary life.

References

  1. Alvesson M (2010) Interpreting Interviews. Sage.
  2. Berinsky AJ (2018) Telling the truth about believing the lies? Evidence for the limited prevalence of expressive survey responding. The Journal of Politics 80: 211-224.
  3. Eckman S, Koch A (2019) Interviewer involvement in sample selection shapes the relationship between response rates and d&and Lucchina, L.A., 2016. The color of emotion: A metric for implicit color associations. Food Quality and Preference 52: 203-210. [crossref]
  4. Western S (2016) Political correctness and political incorrectness: A psychoanalytic study of new authoritarians. Organisational and Social Dynamics 16: 68-84.
  5. Stevens SS (1975) Psychophysics: Introduction to its Perceptual, Neural and Social prospects. John Wiley & Sons.
  6. Luce RD, Tukey JW (1964) Simultaneous conjoint measurement: A new type of fundamental measurement. Journal of Mathematical Psychology 1: 1-27.
  7. De Vos GA, Boyer LB, Borders O (1989) Symbolic Analysis Cross-Culturally: The Rorschach Test. Univ of California Press.
  8. Chalah MA, Kauv P, Lefaucheur JP, Hodel J, Créange A, et al. (2017) Theory of mind in multiple sclerosis: a neuropsychological and MRI study. Neuroscience Letters 658: 108-113. [crossref]
  9. Box GE, Hunter WH, Hunter S (1978) Statistics for Experimenters, New York: John Wiley and Sons 664.
  10. Green PE, Krieger AM, Wind Y (2001) Thirty years of conjoint analysis: Reflections and prospects. Interfaces 31: S56-S73.
  11. Goertz G, Mahoney J (2013) Methodological Rorschach tests: Contrasting interpretations in qualitative and quantitative research. Comparative Political Studies 46: 236-251.
  12. Gofman A, Moskowitz H (2010) Isomorphic permuted experimental designs and their application in conjoint analysis. Journal of Sensory Studies 25: 127-145.
  13. Hultsch DF, MacDonald SW, Dixon RA (2002) Variability in reaction time performance of younger and older adults. The Journals of Gerontology Series B: Psychological Sciences and Social Sciences 57: 101-115. [crossref]
  14. Wason PC (1959) The processing of positive and negative information. Quarterly Journal of Experimental Psychology 11: 92-107.
  15. Dubes R, Jain AK (1979) Validity studies in clustering methodologies. Pattern Recognition 11: 235-254.

COVID-19 and its Impact for Pregnant Women: A Review

DOI: 10.31038/IGOJ.2021422

Abstract

Considering the medical, economic and social importance of the COVID-19 disease in the world, where it is present as indigenous or imported, we have as objectives in this manuscript to contribute to the knowledge of the impact on this viral disease on pregnant women.

Keywords

Coronavirus, COVID-19, SARS Coronavirus 2, SARS-Co2, Pregnancy, Obstetrics, Gynecology

Introduction

COVID-19 is a viral disease whose causative agent was identified in Wuhan-China, as a novel coronavirus, Severe Acute Respiratory Syndrome Coronavirus2 (SARS-CoV-2) [1]. After, 15 April 2020, COVID-19 has caused more than two million confirmed cases and more than 128,000 deaths globally, including 82,295 confirmed cases and 3,342 deaths in China [2]. The Chinese government has locked Wuhan city, since 23 January 2020, and implemented a series of social distancing measures such as: strict traffic restrictions, prohibition of social gatherings; and closure of residential communities [3]. In [4] the authors have referred to “the epidemiological data in China that have shown that most cases had mild symptoms, with an overall case fatality rate of 2,3%. Although, SARS-CoV-2 appears to be less virulent than 2 previous zoonotic coronavirus, SARS-CoV and Middle East Respiratory Syndrome Coronavirus (MERS-CoV), it is far more efficient in transmitting between people in close contact”.

In pregnant women, this novel coronavirus has caused severe complications and both SARS-CoV and MERS-CoV have been found in pregnant women [5,6].

Vertical transmission of this virus has been suspected by several scientists [7], referred to three documented cases of vertical SARS-Co-V2 infection, that were accompanied by a strong inflammatory response. Together, this data supports the hypothesis that in utero SARS-Co-V2 vertical transmission while low is possible.

In [8], we have, in our opinion, a good description of the principal symptoms of Coronavirus-19 in humans, and very accessible for scientist and public in general. In that publication, we found the following information: People with COVID-19 have had a wide range of symptoms reported, ranging from mild symptoms to severe illness. Symptoms may appear 2-14 days after exposure to the virus. People with the following symptoms may have COVID-19:

Fever or chills; Cough; Shortness of breath or difficult breathing; Fatigue; Muscle or body aches; Headache; New loss of taste or small; Sore throat; Congestion or runny nose; Nausea or vomiting and Diarrhoea.

In this publication is observed that “the list does not include all possible symptoms. CDC will continue to update this list as we learn more about COVID-19.”

Concerning risk for COVID-19 disease, are mentioned: old persons; with chronic diseases: heart diseases, pulmonary, neoplasia or arterial hypertension, Other people cited are with the immunological system compromised undergoing chemotherapy, for auto-immunes diseases (rheumatoid arthritis, lupus, multiple sclerosis, or some inflammatory intestinal diseases), human immunodeficiency virus syndrome, or patients with transplants.

Concerning transmission, the authors in [8], have informed that: the data sources were “eligible studies published until May 28, 2020, were retrieved from PubMed, EMBASE, medRxiv, and bioRxiv”. These authors have concluded: “vertical transmission of severe acute respiratory syndrome corona virus 2 is possible and seems to occur in a minority of cases of maternal corona virus disease 2019 infection in the third trimester. The rates of infection are similar to those of other pathogens that cause congenital infections. However, given the paucity of early trimester data, the assessment has yet been made regarding the rates of vertical transmission in early pregnancy and potential risk for consequent fetal morbidity and mortality”.

In [9] the authors have presented “An Analysis of 38 Pregnant Women with COVID-19. Their Newborn Infants, and Maternal- Fetal Transmission of SARS-CoV-2: Maternal Coronavirus Infections and Pregnancy Outcomes 4”. In their manuscript, they have reviewed the effects of two previous coronavirus infections – Severe Acute Respiratory Syndrome (SARS) caused by SARS-CoV and Middle East Respiratory Syndrome (MERS) caused by MERS-CoV – on pregnancy outcomes. On the other hand “analyses were made of the literature describing 38 pregnant women with COVID-19 and their newborns in China to assess the effects of SARS-CoV-2 on the mothers and infants including clinical, laboratory and virological data, and the transmissibility of the virus from mother to fetus. This analysis reveals that unlike coronavirus infections of pregnant women caused by SARS and MERS, in these 38 pregnant women COVID-19 did not lead to maternal deaths. Importantly, and similar to pregnancies with SARS and MERS, there were no confirmed cases of intrauterine transmission of SARS-CoV-2 from mothers with COVID-19 to their fetuses. All neonatal specimens tested, including in some cases placentas, were negative by rt-PCR for SARS-CoV-2. At this point in the global pandemic of COVID-19 infection there is no evidence that SARS [1] CoV-2 undergoes intrauterine or transplacental transmission from infected pregnant women to their fetuses. Analysis of additional cases is necessary to determine if this remains true.”

In [10] The authors have referred: (i) that the disease caused by SARS-CoV-2 was named COVID-19 by WHO and has so far killed more people than SARS and MERS; (ii) in January 2020, the World Health Organization declared COVID-19 a pandemic disease, considering, the widespread global outbreak of COVID-19, with more than 132,758 confirmed cases and 4,955 deaths worldwide; (iii) “Research on both SARS-CoV and MERS-CoV, which are pathologically similar to SARS-CoV-2, has shown that being infected with these viruses during pregnancy increases the risk of maternal death, stillbirth, intrauterine growth retardation and, preterm delivery”; (iv) ”With the exponential increase in cases of COVID-19 throughout the world, there is a need to understand the effects of SARS-CoV-2 on the health of pregnant women, through extrapolation of earlier studies that have been conducted on pregnant women infected with SARS-CoV, and MERS-CoV. There is an urgent need to understand the chance of vertical transmission of SARS-CoV-2 from mother to fetus and the possibility of the virus crossing the placental barrier.”

In [11] the authors have referred to the fact that it was their intention “to review published studies related to the association of severe acute respiratory syndrome coronavirus 2 (SARS-Co.2) infections with pregnancy, fetal, and neonatal outcomes during coronavirus disease 2019 (COVID-19) pandemic in a systematic manner.” In the methods the authors have indicated that “A comprehensive electronic search was done through PubMed, Scopus, Medline, Cochrane database, and Google Scholar from December 01, 2019, to May 22, 2020, along with the reference list of all included studies. All cohort studies that reported on outcomes of COVID-19 during pregnancy were included. Qualitative assessment of included studies was performed using the Newcastle-Ottawa scale” [12].

The authors have screened 513 titles, and included 22 studies, which identified 156 pregnant women with COVID-19 and 108 neonatal outcomes, and they have concluded that “COVID-19 infection in pregnancy leads to increased risk in pregnancy complications such as preterm birth, PPROM, and may possibly lead to maternal death in rare cases.”

Conclusion

  1. We think that it was here demonstrated that COVID-19, has an impact on the health of pregnant women.
  2. We hope that with the attention that is being given to this viral disease is possible, in a short/medium time, to obtain more knowledge, concerning the virus, the treatment and the vaccines, so that with this knowledge is possible a control of all viral variants circulating in the world.
  3. To combat COVID-19, it is necessary:
  • to have persons specialized for the different types of combat;
  • the collaboration between countries at world level;
  • the collaboration of the person, in general, for the execution of the rules stablished by health services of their countries;
  • the collaboration between different governmental services;
  • the collaboration between different community services such as town halls and, hospitals.

References

  1. Archived: WHO Timeline – COVID-19, 27 April 2020 – who.int
  2. World Health Organization. Novel coronavirus – China. Geneva, Switzerland: Word Health Organization httpps.//www.who.int/csr/don/12-january-2020-novel-coronavirus-china/en/. [2020-01-12]
  3. COVID-19 – Global Health, COVID-19: What you need to know about the coronavirus pandemic in 15 April. weform.org
  4. Wuham lockdown: a year of Chinas fight against the COVID pandemic – 22 January, Corona virus pandemic bbc.com
  5. World Health Organization. The epidemiological characteristics of an outbreak of 2019 novel coronavirus disease (COVID-19)- China 2020.
  6. Y Liu, H Chen, K Tang, Y Guo (2020) Clinical manifestations and outcome of SARS-CoV-2 infection during pregnancy. The journal of Infection. [crossref]
  7. Tseng J-Y (2020) Potential implications of SARS-CoV-2 on pregnancy. Taiwan J Obstet Gynecol 59: 464-465. [crossref]
  8. Feuzina C, Biasin M, Catin I, Vergani P, Milet D, Spinilio A, Gismondo M, et al. Analysis of SARS-CoV-2 vertical transmission during pregnancy. Nature Communications 11: 5128. [crossref]
  9. Kotlyar A, Grechukona O, Chen A, Shota P, Grimshaw A, et al. (2021) Vertical transmission of corona virus disease 2019: a systematic review and meta-analysis. Am J Obstet Gynecol 224: 35-53.e3. [crossref]
  10. Schwartz DA (2020) An Analysis of 38 Pregnant Women with COVID-19, Their Newborn Infants, and Maternal-Fetal Transmission of SARS-CoV-2: Maternal Coronavirus Infections and Pregnancy Outcomes. Arch Pathol Lab Med 144: 799-805. [crossref]
  11. Fathi M, Vakili K, Deravi N, Yaghoobpoor S, Ahsan E, et al. (2020) Coronavirus diseases and pregnancy: COVID-19, SARS, and MERS. Przegl Epidemiol 74: 276-289. [crossref]
  12. Hubba Akhtar, Chandni Patel, Eyad Abuelgasim, Amer Harky (2020) COVID-19 (SARS-CoV-2) Infection in Pregnancy: A Systematic Review. Gynecol Obstet Invest 85: 295-306. [crossref]

Experiences of Self-Reported Bullying in Minority Nurses within Acute Care Hospital Workplace Settings – A Grounded Theory Approach

DOI: 10.31038/IJNM.2021214

Abstract

Background: Thirty-five percent of all the known workforce in the United States, across all genders, races, and ethnicities are bullied at work. According to the Workplace Bullying Institute (2017) racial/ethnic minorities in the general population are bullied at a higher rate. By estimating that these trends would continue and remain applicable, racial/Ethnic minority female nurses, as a significant subset of nurses, maybe bullied at a higher rate than Caucasian counterparts. While nurses in the United States (US) who are studied in the health workplace are largely women, female minority wellbeing in the workforce is especially understudied primarily because minority nurses are subsumed in aggregate data that represents predominantly white women. There has been a longstanding goal to increase numbers of minority nurses in all areas of nursing practice but in particular in acute care to advance trust and confidence in quality of care with diverse communities served. The need for minority nurses and wanting to understand the experiences that would draw and keep them in the nurse workforce is a significant area in need of study.

Objective: The objective of this study was to give “voice” to the experience of minority nurses who self-identified as being bullied at work in acute care settings by understanding their experience. Two key areas of exploration were 1) understanding what is experienced and 2) using these data to create an explanatory model that could guide nursing organizations to create a welcoming environment for employment and success of minority nurses.

Design: Face-to-Face semi-structured recorded interviews and survey data including participant demographics and characteristics of professional life and range of support systems in each participant’s personal and professional life.

Setting area hospital(s): The study of nurses currently or formerly employed at Boston, Massachusetts area hospital was conducted at neutral locations outside of the workplace.

Participants: Purposive, typical sampling. Eighteen female minority nurses who work in acute care facilities in the Boston, MA area.

Methods: This study used a constructivist grounded theory method to examine the experiences of self-reported bullying of female racial/ethnic minority nurses in the acute care/hospital workplace.

Results: It was found that organizational racism and discrimination foster an environment where WPB against minorities can flourish. The workplace culture and facility processes appear not to mitigate these circumstances. These mechanisms serve to maintain the status quo and allows those with both formal and informal power to maintain control.

Conclusion: Minority female nurses who are subjected to bullying are forced to conserve their personal resources. They respond by becoming silent about their work conditions or by leaving the job. The consequences of this disengagement by a vital portion of the workforce negatively affects the individuals, the organization, and society.

Keywords

Minority nurses, Workplace bullying acute care workplace culture, Constructivist Grounded

Theory

What is Known About This Topic

. Workplace bullying is a burden on the individual, workplace, healthcare system, and economy

. Minorities are bullied at higher rates than others

What This Paper Adds

. Detailed account of the bullied experience of minority female nurses in the workplace

. A theory of the organizational mechanisms that support and perpetuate workplace bullying of minority female nurses.

The full account of this study resides in the University of Massachusetts Boston Doctoral Dissertation Library.

Introduction

Workplace Bullying (WPB) is intentional abusive behavior that is systematic and repeated to intimidate or control a person, therefore, making them a target [1]. This abusive behavior negatively impacts the individual and the workplace [2]. Bullying involves an imbalance of power, which can be actual or perceived between the target and the perpetrator [3]. Bullying behaviors can manifest as either overt acts such as demeaning in public or covert acts such as undermining. Grimes [4] notes that aggressive, abusive behavior leads to negative impacts on physical health and psychological safety, adversely contributing to poor patient care and medication errors.

The Workplace Bullying Institute conducted a study in 2010 and found that 35% of the workforce across all genders, races, and ethnicities reported experiencing bullying while at work. Within nursing, studies report a wide range of prevalence of 31% to 85%. While bullying can occur anywhere in the nursing field, a study conducted by Vessey and colleagues [5] found that it occurred more often in Acute Care Medical-Surgical areas (23%). The Workplace Bullying Institute found that minority groups are affected by bullying more than other groups and further reported that 39% of Hispanics, 43% of African Americans, 51% of Asians, and 36% of Caucasians are bullied (WBI, 2017). The same study reported that females (66%) were affected by bullying more than males (34%). The sample included 1008 individuals African Americans: 120; Hispanics: 130; Asian: 30; White: 681. Women made up 58% of the WPB targets and; Males 42%. Minorities currently make up 19.2% of the nursing population (American Association of Colleges of Nursing, 2020) [6]. The impact of WPB reaches all levels of society. At an individual level, the target experiences escalating stress levels manifesting into physiological and psychological disorders [7,8]. The effect of individual bullying also reaches the workgroup because the target’s ability to optimize the work unit’s contributions is impaired. For the organization, if bullying becomes embedded into the organization’s culture and multiple departments within an organization are affected, the lack of psychological safety caused by WPB prevents the organization from providing optimal care to all patients under their care. The impact of WBP affects healthcare costs. For example, the exact cost of WPB in the United States is unknown; however, in England, the estimated cost of workplace bullying is estimated to be around $3 billion/year [9]. In 2017 the United States spent 17.9% of the total Gross Domestic Product ($3.5 trillion or $10,739 per person) on healthcare. Being mistreated at work lead to a 42% increase in missed workdays in a sample size of 13,807 employees [10] calculated this to be around $4.1 billion. A prominent factor in healthcare costs is health disparities [11]. Since it is conjectured that WPB disproportionally affects minorities and nurses, the phenomenon of WPB becomes a barrier to recruitment and retention to address the factors that lead to health disparities. Retention and recruitment suffer, which may contribute to Hospital turnover, which is on the rise, currently standing at 19.1% [12]. Themes emerge from a review of the literature specific to bullying of minority nurses. First, minorities get bullied in the workplace at higher rates than others. Second, race/ethnicity is a stressor and agonist for WPB [13-15], and third institutional racism permeated the workplace. Workplace characteristics and ethnicity were predictive factors for bullying because it signaled to the workers that the prevailing culture is the dominant culture [14,16]. As a result, minority groups lived in fear of ongoing attacks and or anticipating attacks [17]. There was fear of retaliation. The theory of social identity suggests that minority groups are likely to be targets of bullying due to differences in appearance, communication style, and assimilation into the popular culture [18,19].

Nurses belonging to multiple minority groups experience bullying more than those nurses who only fall into one category of the racial-ethnic minority. Female minorities face challenges not necessarily faced to the same degree as male minorities.

Two questions emerged from gaps in knowledge from the literature:

1) What are the experiences of racial/ethnic minority nurses with workplace bullying? 2) What are the antecedents and consequences of WPB on racial/ethnic minority nurses? A constructivist grounded theory methodology aided in answering these questions.

Method

Constructivist Grounded theory is a qualitative research methodology that seeks to understand a social process where no adequate prior theory exists. It uses an inductive approach to generate a new theory. The theory culminates ideas and constructs operationalized by social structures and processes [20]. Constructivist grounded theory is a useful method to examine individual experiences with workplace bullying to uncover themes to explain and delineate the participants’ experiences.

Participants

The Purposive (typical) sampling technique enabled the recruitment of racial/ethnic female minority nurses who worked in acute care hospitals in a metropolitan city on the east coast of the U.S. (within the last year). Recruitment occurred through word of mouth and posting of recruitment flyers on sites like LinkedIn and Facebook. Nurses were eligible if they met the above criteria and which included: spoke and read English at a 10th-grade level; phone access; employed in an acute care hospital in the Metro Boston area either full or part-time; were aged 21 years or greater; identified as a racial/ethnic minority by definition; had access to a primary care provider. Excluded were those who presented with cognitive impairment, any bullying experience over two years ago, vulnerable populations, males (including minority male), and Caucasian females (see Table 1 Sample and Demographics).

Ethical Review

The study procedure included obtaining Investigation Review Board approval at the University of Massachusetts Boston.

Procedure

Communication with participants first involved the establishment of eligibility and willingness to participate. Next was a review of WPB’s definition and the determination of self-reported racial/ethnic minorities. If eligible, they filled out a written consent, demographics, and other participant information utilizing Qualtrics. A 60–90-minute interview was then scheduled and accomplished by the primary researcher in a suitable location. The interview was audio-recorded and transcribed into a sanitized written transcript. At the completion, they received a $25 gift card funded through the primary researcher’s resources. No outside funding sources contributed to this research. The final communication with participants involved a written brief of the study findings with an offer to discuss the study findings via a phone call. Throughout the study, a reflexivity journal was essential, considering the nature of the content discussed. With the negative emotion often generated, it was necessary to reflect on the researcher’s objectivity. Analysis of the memo log and entries were consistent with the process applied during coding. Memos allowed an overall perspective and guided the putting together concepts and linkages from the data. Memos were crucial to developing the subthemes, hypothesis, themes, and the theory that resulted.

Data Analysis

The data analysis process consisted of coding, comparing, memo writing, sampling, and theory production [21]. The process of coding involved an iterative process of initial coding and focused coding. During the initial open coding stage, the primary researcher reviewed data word by word and line by line. Probing questions guided the understanding of what was emerging from the data. These questions guided modifications to future interviews and coding. Two such examples center on the extreme negativity of the WPB experiences of the participants. One was the need to establish neutral, open coded terms where possible. Another example was to ask the participants if anything good came out of their experience. Research team members reviewed and discussed the coding throughout the coding and analysis process. For this study, a priori sample size initially was set at twenty interviews. Theoretical saturation was determined when three consecutive interviews gave no new concepts. Sampling continued to the point of terminal saturation. The sample size was adjusted [22] to eighteen after no new concepts emerged after the tenth interview. Data saturation was proved with no new concepts emerging after ten interviews however since eighteen interviews were completed at this time all data was included for analysis. An increase from 245 open codes after ten interviews yielded 13 initial axial coded groups or categories. There were no new axial coded groups or categories when increased to 395 open codes after 18 interviews. The categories were adjusted a couple of times, but there were no new meanings encountered. The constant comparison method [19] compared data with data, data with codes, and codes with data. The initial codes were open for modifications, but at the end of this stage, codes that are most frequent and or significant are identified, leading to focused coding where a review of the larger data segments took place. The data formulated into temporary conceptual positions with the application of the framework of axial associations. Data analysis software (NVivo 12) served as the base reservoir of the transcribed data, and Microsoft Excel was the vehicle for the data sorts. Theoretical coding involved analyzing the memos [23] to connect different categories and codes. The constant comparison method aided by the memos’ resulted in a final re-sorting and realignment of the concepts. Eleven final categories emerged, making up three major themes and three transitional or linking categories. Hypothesis aided in understanding the relationship between categories. From this process, the themes and theories were developed and solidified.

Findings

This study consisted of eighteen participants. Table 1. Presents the Demographic data. Participants included seven African/American; five Hispanic American; three Asian American; one Native American, and two identified as “other.” The participants represented a span of work experience. More than half of the participants were married or in committed relationships.

Table 1: Demographics

Gender

Number

Percent Range

Mean

Female

18

100

Race
African American

7

39

Asian/Asian American

3

17

Hispanic/Hispanic American

5

28

Native American

1

6

Other (English as a Second Language)

2

11

Marital Status
Married

13

72

Widowed

3

17

Single

2

11

Age
20-29

0

0

30-39

3

17

40-49

8

44 25-65

47.3

50-59

4

22

60-69

3

17

Educational Level
Doctoral

2

11

Masters

5

28

Bachelor

10

56

Associate

1

6

Diploma

0

0

Employment Status
Full Time

18

100

Part Time

0

0

Practice Area
Med/Surg

5

28

ICU

1

6

Prenatal

1

6

Other

11

61

Position Held
Staff

10

56

NP

1

6

Supervisor

0

0

Administer

6

33

Other

1

6

Employment Years
1-3 Years

3

17

4-6 Years

1

6

7-9 Years

1

6

10-12 Years

2

11

>12 Years

11

61

Static/Float Role
Static

16

89

Float

2

11

Characteristics of the participants related to their professional life and the range of support systems in each participant’s personal and professional life are in Table 2. The personal support system includes four types of supportive behaviors: emotional (expressions of empathy, love, trust, caring), instrumental (tangible aide), informational (advice, suggestions, and information), and appraisal (assists with self-appraisal) [24]. Seventeen participants reported receiving personal emotional support, with 13 (72.2%) listed as very supportive, while only 3 of 18 (16.7%) received very supportive professional help. None reported personal nonsupport. Six out of 18 (33.3%) did list professional support as unsupportive.

Table 2: Support Systems

Support Type

Number

Percent

Emotional

17

94

Instrumental

9

50

Informational

14

78

Appraisal

10

56

Personal Support Rating
Very Supportive

13

72

Somewhat Supportive

5

28

Neither Supportive now Unsupportive

0

0

Somewhat Unsupportive

0

0

Very Unsupportive

0

0

Professional Support Rating
Very Supportive

3

17

Somewhat Supportive

7

39

Neither Supportive nor Unsupportive

2

11

Somewhat Unsupportive

3

17

Very Unsupportive

3

17

A rich database from the interviews yielded the following results. Table 3 revealing participant’s reflection of what the experience meant to them and how they dealt with the effects of WPB. The selected samples of direct quotes from participants have been organized around three themes with the categories as links, merging to form a theory.

Table 3: Data Analysis

Themes GROUPS Axial Association
Organizational Injustice Racism A, B
Discrimination
Exclusion
Transition Opportunity C
Organizational Stability that Fosters WPB Facility Process C, D
Workplace Culture
Management Competency
Transition WPB Acts A
Survival Effects of WPB E, F
Coping
Transition Back to Organizational Stability Silence F
Phenomena that relates to the actions and interactions of WPB A
Causal conditions that resulted in the occurrence of WPB B
Attributes of the context of the WPB C
Intervening conditions that influence WPB D
Actions and interactional strategies participants used to cope with WPB E
Consequences of actions and interactions F

Organizational Injustice

First is the theme of organizational injustice, defined as instances of employees perceiving organizational actions, policies, decisions, or messaging to be unjust or unfair [25]. These are instances where employees perceived that they were mistreated compared to other employees, including actions that stemmed from feelings of superiority, inequality, or racism. The theme of organizational injustice includes three subthemes of racism, discrimination, and exclusion. Examples from the data representing these three subthemes follow.

Racism: A participant described a coworker whose actions conveyed aggression and hostility based on the participant’s race. The coworker’s actions conveyed racism. A participant noted:

“Comments would be made about anybody that’s of African American race; that they were dumdums and slow. Whenever there is African American or black nurses or nursing assistants in the classroom, she would say they will never get it; they are not going to get it. Those people are always slow. She said black people are known to be slow. She said those very words.”

Discrimination: A participant observed selection for positions based on race:

Right. So, the group of people that are getting these positions, and that can exercise this power. They are all white, But if it is a white person, then things get a little bit smoother, so you know what is going to happen based on the color of the nurse.”

Exclusion: The race or ethnicity-based discrimination and exclusion experienced by these participants is ubiquitous and ongoing. When individuals work for organizations that exhibit organizational racism, they can feel isolated or excluded from cooperation, social, training, or advancement opportunities.

Another participant started with a classic example of suppression of opportunity when directed not to present a project she had completed for her work unit:

“My assistant manager even did not want to allow me to present it. Wow, who is now still there? So, after the meeting, she said, ‘Oh, who wants to hear what N4 has to say.’ She was reluctant to allow me to present.”

The three subthemes are deeply interconnected, as organizational racism can be the root cause of both discrimination and exclusion. These data revealed the possibility that discrimination may occur throughout the organization during employee hiring, salary negotiations, training opportunities, and consideration for advancement. Discriminating against individuals in a minority group results in their exclusion from opportunities. The exclusion occurs via a deliberate “control of opportunity,” leading to the second theme maintaining the status quo.

Organizational Characteristics that Foster Bullying

The second theme relates to organizational characteristics and processes that foster bullying of female minority employees. There are three subsections of the facility process, workplace culture, and management competency. Many participants discussed the lack of resources to help with the experience of WPB. These resources include collective bargaining (union activities) and other employee assistance entities and the lack of management ability to understand and mitigate the situation. The notable facility factors highlighted to be inadequate included ineffective advice or assistance by employee assistance entities.

Regarding the effectiveness of the Union and other facility entities, a participant said:

“They implemented a couple of years ago, maybe last year,… a website… an anonymous website that you could go in. You do not have to go to your manager because we figured that the manager does not help, and H.R. does not help, and the Union does not help”.

When facility processes fail to result in productive actions, participants report it being a barrier to reporting problems. A participant illustrated how limitations in time constraints are a barrier to reporting issues:

Because EEO, at this hospital, you have to file a case within 21 days. It seems to me everywhere it is more than a few months. My lawyer had to write them, and they should excuse the time limit. Anyway, the system they have in place is rigged.”

Next is the workplace culture, which exists within the context of racism, discrimination, and exclusion. Workplace culture includes the subcomponents of teamwork, workload inequities, cliques, and informal power.

A participant stated that some managers found bullying to be humorous and were unable to take behavior issues seriously:

“There was another scrub he did not like because he had an accent. They had a physical fight. This is the culture I was in. When the manager came in, she was smiling, not taking it too seriously. It was fun for them.”

A participant demonstrated the normalization of bullying in the workplace:

“She got away with it because nobody was reporting it. I guess that was a cultural norm from what I heard, and that is how they always are. So, it was like cultural acceptance, a cultural norm”.

In the workplace, the objective of teamwork is to support the outcome of the work product. Cliques in a workplace, however, are formed to meet the individual needs of the workers. Participants in this study frequently spoke of race/ethnic-based cliques, which develop out of the need to improve a sense of control or power over their environment.

A participant described a clique at a granular level and how it impacts teamwork and team reliance.

“So you just get those other black nurses to help you. I remember days like; you come in ‘oh my God, I hope there’s somebody that looks like me. I hope there’s another black person here.’ And that’s what we are always saying under our breath.”

Workplace culture comprised accepted and repeated behaviors because they served some function that maintains the existing structure, even if those behaviors are detrimental to some people. Considering the workplace culture, its influence in perpetuating WPB is evident. Inasmuch, it is essential to look at the processes within the healthcare institution’s culture and structure, including management, leadership, and limitations of opportunities for participants. Informal power emerged as a critical component of workplace culture. Informal power is that which results from relationships that develop in the workplace. Informal power is obtained from relationships that employees build with each other and may come from additional influences such as intimidation, fear, and self-entitlement [26]. When a group of lower power individuals is combined with one of higher power the outcome of work teams suffers. The group begins to feel vulnerable and becomes aware of the power inequity raising intra-team power sensitivity. Resource threats in the group such as assignments, workload, unequal application of tardiness rules, and external resource threats such as budget cuts and layoffs bring forward intra-team struggles. This dysfunction results in adverse outcomes for team performance, organizational efficiency, and patient care [26].

Employees with no formal authority were able to wield influence over other employees supported informally by the workplace. Participant said:

I did have a coworker like that. It was kind of interesting because she was a friend with a couple of managers. And she would talk about how, ‘Oh, I’ll just see her tonight. I’ll talk to her later, regarding….’ She would always give you the impression that they were going out for drinks after work.”

The third of the three subthemes is management competency. Many participants do not directly challenge management’s competency; however, analysis of remarks points to this as a factor in their WPB experiences. Managers are responsible for the work environment that optimizes the quantity and quality of work accomplished [27]. The failure to create this environment can negatively impact the employees’ well-being and health and the work output. The loss of credibility of the manager based on their actions is an example of this. One participant stated:

“Earlier on, there had been a case where people had been stealing time, and it came to fruition with the awareness of Central Office. And they did an investigation that they charged that manager and her assistant with manipulation and stealing of time.”

Evidence that management is unwilling or incapable of acting appropriately or doing the right thing is the source of participants feeling disheartened and discouraged. A participant said:

“I tried to inform the Chief Nurse. That is when I realized that she might be part of it. Because nothing was ever done. I went to her when it happened. And then it was like what I told her, somehow was worked into my [doing the] bullying”.

Specific to race, ethnicity, and management cultural competency, Participant illustrated a management deficit: “So, I think it was a lack of understanding of me as an individual and a lack of respect of me as an individual.”

Some participants reported experiencing instances of their managers “gaslighting” them. The term “gaslighting” refers to one individual’s ability to erode the sense of another’s reality (Arabi, 2019). One participant spoke of staff that got together to come up with a typical story regarding a conflict at work:

“The manager says, ‘I need to hear from his side.’ And then the surgeon and other nurses got together and wrote me up. So, that is not the first time the surgeon demanded, and they all agreed. So, they said they all kind of coordinated. And the manager was very happy to get them on her side.”

An essential part of “gaslighting” and worthy of separate mention is false accusations. These set the stage for building a manufactured narrative about a target of WPB. A participant addressed this when accused by a supervisor of keeping a personal logbook about people’s activities in her office:

You can call the police right now and have them search like there’s nothing there. It’s not true. Then I found out from another colleague that everybody had been asked about their interactions with me. So, I was asked to leave, and I was told there’s a GYN position, right, you can take. And the irony of it was at the beginning of that same meeting; I was accused of refusing to see female patients.”

Survival

Last is the theme of Survival, which includes two subthemes: Effects of Workplace Bullying and Survival. Effects of workplace bullying describe the lived experience of female minority nurses regarding the reported WPB effects and serve as a validation that participants‘ descriptions of their experience correlate well with existing literature regarding the effects of WPB. Fifteen of eighteen participants interviewed for this study reported suffering from anxiety and stress. A participant said,

“I think… at first it was just…it started as nervousness and a little bit of anxiety of like, ‘Oh, I have to be perfect and like do everything right.” Another participant explained:

I kept records, and when I went to report it initially to the Director with the first incident, and he asked me if I wanted to go back to my department. And I said ‘no,’ because I couldn’t even walk down that hallway without getting a dry mouth and having my heart race.”

Another consequence of WPB is the effect of dehumanizing the victim. This aspect is often associated directly with the person’s ethnic or racial minority status. A participant experienced the following where a coworker deliberately referred to her by the wrong name:

“I will tell you what happened one day, the same surgeon that I told you who physically grabbed me…called me Natasha. He would call me Mila one day and Natasha, making fun of me and making jokes about my accent. And one day, I will never forget, he went around and said, ‘Look Mila’ and I said, ‘I am Natasha.’ So, I tried to make some jokes to get through this, but you get fed up with this.”

A Native American participant revealed that the impact of workplace bullying was demoralizing and dehumanizing:

“I remember going to the ‘bed meetings,’ and my counterparts would make the whooping noise to make jokes about Native Americans, and I just ignored them. And I didn’t realize that they were in the process of demoralizing and dehumanizing me.”

The effects discussed above demonstrate that these participants went through matches the effects and consequences of WPB, as reported in the literature. What is more, these events show context for their overall experience and why they had to attempt to cope with their lives and livelihoods.

Survival includes examples of coping. A significant finding of the study was that when minority female nurses are in workplaces where bullying routinely occurs, they can reach a point where they acknowledge that the bullying is unlikely to stop or change, regardless of their actions. Every participant had statements coded that related to coping. The interview results related to coping included emotion-focused coping, referring to the regulation of emotions generated by bullying events like anger or frustration, and problem-focused coping, referring to an effort to solve or mitigate the problem or avoid the problem in the future. It was clear from the following results that problem-focused coping far outweighed emotion-focused coping.

For one participant, the emotion-focused response was to cry, which represents a release of sad, frustrated, or otherwise negative emotions,

“And then after that, I start to cry because it’s, you know, bullying and bullying, bullying, I think that crying is the best thing. But I cry so heavily, you know, I felt like, how am I going to work tonight?”.

Prayer and other spiritual guidance aspects are the most frequently cited example in the emotion-focused coping category. Prayer is well established in human history as a mitigation strategy for stressful situations, and this continues into these examples from bullied minority female nurses in the workplace. A participant described using prayer and faith to get through the day: “Praying works for me all the time. So, it’s the prayer that’s kept me at work up to today. It works for me. I tell you 100% yes to that. And that’s what gets me through.

The consistent exposure to the WPB experience and lack of assistance from any external source left the participants working out how to survive their receiving treatment. Having no control over external factors, they turned to an internal factor they could control: to go silent about the treatment and or leave their position.

Problem-focused coping was concentrated on the strategy of silencing-the-self or leave the job. All participants made remarks about leaving the job where bullied, going silent about the bullying, or both. There is no ambiguity with many of the WPB participants driving their intention to leave that job. A participant stated: “Oh yes, right, this is why a nurse left the ward. I become the next target. And I decided to leave. This is why I left because you don’t attack me professionally.”

A response from another participant showed the desire to get away from the bully, “So, I knew the only way to get out was to apply for a different department where she would have no control.” When the interviewer asked, “What do you think would have happened if you had to stay in the same position?” her response was, “I knew I was going to quit.”

Repeated WPB experiences were behind a participant leaving other jobs as well:

I’ve left jobs because of bullying. I left the ICU position because I didn’t have ICU experience. I wanted to learn the ICU, and I had a nurse there, she was my preceptor, and she was just so, so mean and nasty.”

In the absence of mitigating circumstances, the WPB experience’s effects led directly to all eighteen participants’ decision to go silent and or leave the position where bullied.

Discussion

The purpose of this constructivist grounded theory study was to examine the overall experiences of self-reported bullying of female racial/ethnic minority nurses in the acute care/hospital workplace in their own voice through dialogue. Three significant themes describe the antecedents (cultural injustice), contributing factors (organizational stability), and the consequences of bullying (Survival) for the participants. Components of workplace bullying reveal a workplace environment and facility processes that synergistically form the context for experiencing bullying. Workplace culture, the overall work environment, the lack of teamwork, and the formation of workplace cliques represent the structure that makes workplace bullying possible. The participants described the processes that allow WPB to occur, including leadership passivity, lack of facility resources to support those experiencing WPB, deployment of informal power through favored persons as perpetrators of bullying, the vanishing of professional opportunities for those targeted, and sabotaging the quality of work of minority nurses. Ineffective organizational oversight was described that allowed workplace bullying to occur repeatedly while bullying and various coping strategies used against it resulted in self-silencing or leaving the job. The current literature on workplace bullying indicates that racial or ethnic minorities experience bullying more than non-minorities (WBI, 2017). However, contemporary literature lacks sufficient rigor on the racial/ethnic minorities’ perspectives and the circumstances surrounding their experiences. The purpose of this study was to bridge the gap in the literature by examining the experiences of female nurses who self-identify as racial/ethnic minorities that have faced bullying in an acute care hospital setting. This study aimed to understand how these experiences are unique to racial or ethnic minorities and describe specific factors that give rise to these unique experiences using a rigorous type of research inquiry. Understanding these unique bullying experiences and addressing the factors leading to bullying of racial/ ethnic minorities in acute care settings will have numerous benefits to society. The literature illustrates that health outcomes improve when nurses’ racial and ethnic makeup represent the communities they serve. However, if these same nurses are being bullied at higher rates, stripped of their voice, the attrition rate will outpace the replacement rate. The quality of care received by the corresponding patient population cannot improve. Understanding this group of nurses’ workplace bullying experience is essential for creating a supportive place to work. Three themes emerged from the data. These three themes and their subcategories describe the workplace bullying experiences of female minority nurses. They also describe the organizational factors that propagate and perpetuate bullying of these nurses. Figure 1 illustrates these mechanisms. This figure illustrates the mechanism of the perpetuation of workplace bullying in the nursing workgroup. From this view, we can focus on the role of organizational culture and the finding that within an environment fostered by the workplace culture, management competency, and facility process are the breakdown of real teamwork, the rise of cliques, and the inevitable workload inequities that result leading to the acts of WPB. There is a breakdown of collegiality and a lack of a common goal impacting the organization’s output. Coping with silence or leaving the position was the universal result.

fig 1

Figure 1: Cycle of Workplace Bullying of Minority Nurses

The three themes and their relationship with each other revealed the core social process of bullying, maintaining the status quo through the deliberate attraction-selection-attrition of employees. Bullying maintained the status quo by perpetuating a culture where anyone who did not fit in with the dominant group of the organization was left with a choice to leave the job or become silent. Racism, discrimination, and exclusion make up the theme of organizational injustice, which results in the unfair allocation of opportunity for minority nurses. Racism and discrimination are foundational in all workplace settings, but the degree and how it is manifested may differ. Organizational injustice forms the backdrop and is a precursor for elements within the workplace culture to include facility process and management competency that did not function in a way that could stop or even significantly mitigate these actions. Facility processes were not effective mechanisms for surveillance, to assess the overall workplace culture, or to manage reports of WPB occurrences. One explanation for this could be the attraction and selection of employees that match the status quo in concert with the attrition of the minorities viewed as outliers. The victims of WPB had two options: to leave or be silent if they stayed. In this study, silence or to leave was functionally the same action as either helps maintain the status quo. Those who did not fit in could and did push back but only to the extent that they had personal and professional resources available. These mechanisms describe the theory of structural-functional bullying in nursing Figure 2.

fig 2

Figure 2: Maintaining the Status Quo

Academic literature supports organizational injustice findings [28,29] stated that racism is the initial framework from which discrimination and exclusion can build. In a racist workplace, individuals are far more likely to be excluded and discriminated against [28,29] found that racism by individuals with hiring authority reflected the type of employees they select and to whom they provide promotional opportunities to. At an institutional level, racism becomes depersonalized. The lines between racism, bullying, and discrimination became blurry, and the inability to hold any person or entity directly accountable makes it challenging to address [30]. Workplace Characteristics that Promote Bullying, the data analysis shows that participants experienced flaws in facility processes such as confidential reports being shared with subjects and unions or human resources representatives failing to follow up on bullying reports. Additionally, workplace culture elements fostered bullying and occurred in a spectrum of minimizing complaints to enabling an outright hostile work environment. Participants reported a culture of employees protecting themselves and securing an environment that unfairly blames some employees. Finally, participants reported experiencing issues with managers who lacked credibility or managers who relied on bullying to force employees’ compliance. The attraction and selection of like-minded people that fit the dominant culture and the attrition of those that do not fit in explain the mechanism for workplace culture formation. The Attraction-Selection-Attrition model explains that organizational culture, and the social structure that emerges from it, is built and formed over time by the individuals within that organization. Facility processes such as the Union or other employees’ resources were inadequate to address reports of bullying. In theory, facility processes were in place to improve employee fairness, satisfaction [31,32]. Two typical facility processes in play here were union support for the nurse and the facility process for reporting issues detrimental to the organization and its people. The findings, however, showed little to no positive outcomes of these processes.

Managers in charge of workgroups were unable to mitigate bullying. Managers were recruiting bullies to collect information and bully others. They thrived in a culture of chaos where the employees were fighting with each other. Lack of cultural awareness and management competency may be an agonist for the prevalence of WPB against minorities.

Factors working together made up the workplace structure and culture, including the global environment, management competency, and cliques considered teamwork. These factors, when combined with facility process factors, set the stage for acts of WPB. Organizational complicity allowed the cliques to flourish, permitting discrimination and informal power by individual staff-level employees. Bullying action was allowed within this context, including tactics of gaslighting, false accusations, favoritism, exclusion, and undermining. These structures and techniques tended toward organizational stability though favoring the status quo. WPB was not inhibited, which in turn perpetuated a climate of bullying. The workplace culture that came forward in this study demonstrated how the current environment empowered bullies. Also, the same processes channeled bullying acts because bullies were protected. The result squelched opportunities and destruction of professional dreams that remained out of sight until the individual stories brought it out. The workplace culture demonstrated a contradiction between a caring environment’s expected nature and surface civility, with the participants’ aggressive, non-caring behaviors. As a result of the workplace characteristics, bullying acts were allowed to go on, causing participants to go into a survival mode as they tried to cope with the effects. Consistent with appraisal theory, Survival involved both emotion-focused coping and problem-focused coping. Resulting actions included deliberate attempts at resolution from which silence and leaving became the most common form. The nurse’s choice to consciously become silent seems to result from the desire to control the situation by conserving resources. The concept of resilience can be a factor for participants going through these traumatic events. On the surface, resilience is a term used to denote someone exposed to challenges but can adapt and move on. In biological theory, it involves the concept of natural selection and the phrase “survival of the fittest” [33]. The implication is those who should survive do end up surviving. Resilience [34] can have a positive connotation. Our society values this trait in people [35], but this view does not adequately explain these participants’ experiences with bullying and its aftermath. The self-silencing is not a form of resiliency and does not demonstrate acceptance. The silence is a deliberate effort to conserve resources. Self-silencing represents a functional mechanism to carry on. This study has shown that nurses may be erroneously viewed as “resilient” when, in reality, they may just be avoiding the “stigma” associated with getting bullied. This form of silencing occurs due to the limited energy available to minorities to counter the repulsive force and change the status quo. The minority nurse’s operational decision was to conserve resources and rely on personal support mechanisms to carry on. The self-silencing manifested in ways that were not mutually exclusive, including silence about how one was treated at work in their current position and being silent while leaving the job to take action against bullying. The nurse’s choice to consciously become silent seemed to result from the desire to control the situation by conserving resources. It emerged that self-silencing was mutually beneficial in the short term for both the participant and the organization. In contrast to the individual level experience of self-silencing, maintaining the status quo perpetuates the workplace culture. The status quo is supported by those who bully and self-silence or leave the job. In discussing the themes, another relevant concept is the concept of intersectionality. Kimberlie Crenshaw defines intersectionality as how race, class, gender, and other individual characteristics “intersect” with one another and overlap to influence societal and interpersonal interactions [36]. Intersectionality requires that consideration of the uniqueness of each individual and their personal experiences. As intersectionality became more complex for the participants (i.e., female, black, religious minority), the likelihood of being mistreated from all sources grew. This study’s revelation, needing further exploration in quantitative studies focusing on isolating related variables, separates the perceived impacts of race, gender, and unrelated personal quality on workplace bullying. Many individuals in the study perceived bullying instances to have a racial component, but others may have included a gender component. Women face bullying in the workplace, and individuals belonging to minority groups experience bullying in the workplace, so that those female minority nurses may experience bullying differently [37].

Implications for Policy

Health inequities and health disparities are a significant economic burden on government. For example, while the healthcare costs account for 17% of the gross domestic products, the health care quality in the United States is the lowest among all other industrialized nations [38]. With the increase of minorities in the U.S. population, examining minority nurses and the workplace’s specific challenges is essential in our society’s evolution. Minorities will soon become the majority of the U.S. population. Reducing WPB against minority nurses has ramifications well beyond the proper treatment of individuals. Evidence in the literature shows that improving the influx of minority healthcare workers may remedy the growing health disparities. To retain minorities in the healthcare space, federal governments, and organizations could consider instituting an anti-bullying framework. It is important to frame these findings with the quadruple aim of the Institute of Healthcare Improvement (IHI) for the future success of health care systems worldwide (IHI 2017) [39,40]. In keeping with IHI’s triple aims of excellent quality of care at an optimized cost while improving the health of the population served, one would argue that bullying’s organizational support provides a fertile ground for bullying to occur. Care suffers for both the nurse and the patient, increasing medical errors and decreasing the nurse’s sense of self. Increased turnover and other errors drive up the cost of care. This environment undermines the trust between the frontline nurse, the administration, and the public they serve. IHI’s fourth aim involves improving the work experience of healthcare workers. It links patients’ healthcare experience to the nurses’ overall work experience [41], underscoring the urgency of developing WPB mitigation strategies. Strategies could start with resources to improve cultural competency, focusing on implicit bias, multiple reporting avenues, awareness and training, building a functional support system, and assessing practical teamwork barriers in nursing. It is reported in the literature that many employees are afraid of reporting bullying incidents for fear of reprisal [42,43]. Multiple reporting avenues provide options for employees to select the reporting mechanism with which they feel most comfortable. Anonymous reporting mechanisms should be made available. Many employees perceive that their workplace structure supports a bullying culture [3,44]. It is essential to start, however, to build a culture of trust for employees. Awareness and training are a start to this. As organizations initiate intervention strategies, it is essential to note that reported bullying’s initial frequency and intensity may increase due to heightened awareness of bullying [45].

Study Limitations

This study has several limitations. One limitation is inherent to qualitative studies, in that it is not able to establish correlation or causation between specific variables. The participant sample for this study was appropriate for a qualitative study but too limited to be representative. The findings in this study are not applicable to all nurses, all female nurses, or even all minority female nurses. To establish causation, further studies would need to conduct randomized control trials which are representative of the population they include. However, the workplace factors such as cliques masquerading as teamwork can form the basis for intervention research utilizing model for improvement framework. Similarly, another limitation is the focus on female-only population. While the importance to understand the experiences of all groups of employees to isolate all factors that make up the multidimensional nature of WPB was perceived, this study focused just on female minority nurses due to their exposure to the microcosm of societal and organizational factors that are unique to female experience in a male-dominated society. These dominations operate to silence their voices. Furthermore, even within female participants, there are cultural variations within minority groups that could not be fully explored in a pilot study of this size. In order to fully understand the experience of minority nurses with workplace bullying, it would be important to compare male perspectives and identify areas of similarity and differences. Another limitation of this study was a lack of focus on individual attributes and how they interact with their coworkers. There are many factors which contribute to workplace bullying, including cooperation style, communication style, individual preferences, and inherent characteristics like race or gender. Without understanding the characteristics of the individual nursing being bullied and the individual doing the bullying, it is challenging to understand why bullying occurred and how it could be prevented in the future (Pallesen et al., 2017). Further studies could consider isolating variables which contributed to bullying, which may include the intersectionality of race, gender, and ethnicity and the WPB of nurses.

Conclusion

The constructivist grounded theory method provided a framework to guide participants in examining their bullying experiences in the acute care workplace. A significant finding was the extent to which racism and its companion concepts of discrimination and exclusion are connected. The summation of organizational processes and the participants’ use or non-use of resources revealed a workplace culture focused on maintaining the status quo. Three factors that supported this were workplace culture, management competency, and facility processes. Resistance from those that did not fit-in occurred only to the extent that they had personal and professional resources available. Many participants discussed the lack of resources to assist them in dealing with the environment. All participants made remarks that consisted of either leaving the job or going silent about the bullying. The consistency of such comments across participants clarifies that these coping-related responses, leaving the job or silencing, were crucial aspects of their WPB experience and how to carry on in its aftermath.

This study’s results support the Institute for Healthcare Improvement’s Quadruple Aim Initiative to improve the health of populations, enhance the experience of care for individuals, reduce the per capita cost of health care, and in particular increase the quality of the work experience for healthcare providers by allowing them to attain joy in work (IHI, 2017). Workplace psychological safety and the essential component of cultural competency are integral to improving, rebuilding, and reshaping workplace culture. By promoting cultural competency within its structure and processes, an organization could create a psychologically safe environment for all employees. Also, focusing on attaining joy in the workplace through psychological safety would create an environment where minority and all nurses would want to work, succeed, and flourish.

Funding

No external funding was used for this research.

Conflict of Interest

There are no conflicts of interest with the authors of this research.

Interview Guide

1. Tell me about your experience with workplace bullying?

2. The Bullying that you experienced: Was it an attack on you, personally, against your professionalism/competence, or against you by affecting your work tasks?

a. Personally

Did you have a personal relationship with the bully?

Did you or the bully try to have a personal relationship with each other?

Did the bully ever directly threaten you?

What do You Feel Now?

Are You Able to Move On?

b. Against your professionalism/competence:

Were there attempts to discredit you? Give examples

Do you feel that this has impacted your professional career?

Do you feel that this has impacted your personal life?

Do you feel that this has impacted your health?

c. Against you by affecting your work tasks:

3. Were there attempts to limit or otherwise interfere with your work? Give examples

4. Did this make you change anything you did? How?

5. Upstream Factors

a. Did your workplace tolerate behaviors consistently?

What were some examples of differences?

How did that make you feel?

What if anything, did you do because of this?

6. Did your workplace reward staff consistently?

a. What were some examples of differences?

How did that make you feel?

What if anything, did you do because of this?

7. Did you observe cliques in your workplace?

Did cliques have influence over decisions that affected your work there?

Did you feel pressure to join a clique?

Did this change anything you did or didn’t do at work?

Did this change how you felt at work?

8. Did you observe people attempting to exercise more authority than you thought they had?

a. Was this known to higher authority?

Did higher authority tolerate this?

Did this directly affect you at work?

Did this indirectly affect you at work?

9. Did you observe people at work ever deliberately violate known policy or procedure?

a. Did it affect your work?

Did it affect you?

Did it make you change anything you did at work?

What do you feel now?

Are you able to move on?

10. The bullying you experienced at work, do you feel it affected or changed anything in your life outside of work? Please tell me about that.

11. Has this impacted your personal life (can you explain)?

12. Downstream Factors

a. How do you cope with getting bullied?

b. Do you think getting bullied affects your ability to provide safe patient care?

c. Can you explain?

d. Has getting bullying affected your health?

Examples include: (anxiety, depression, psychological and physiological changes)

Have you had to seek medical or psychological treatment?

13. Reporting

a. Did you report the bullying to anybody?

Do you feel comfortable reporting bullying incidence at work?

Did you notice any consequence to you reporting bullying?

References

  1. Workplace Bullying Institute (2010) Workplace bullying: Recognize and prevent it.
  2. Askew DA, Schluter PJ, Dick ML (2013) Workplace bullying—What’s it got to do with general practice? Aust Fam Physician. 42: 186-188. [crossref]
  3. Hutchinson M, Vickers M, Jackson D, Wilkes L (2006) Workplace bullying in nursing: towards a more critical organizational perspective. Nursing Inquiry 13: 118-126. [crossref]
  4. Grimes-Holsinger V (2018) Reduction of workplace discord through self-awareness and personal de-escalation skills [unpublished doctoral dissertation]. Quinnipiac University.
  5. Vessey JA, DeMarco RF, Gaffney DA, Budin WC (2009) Bullying of staff registered nurses in the workplace: a preliminary study for developing personal and organizational strategies for the transformation of hostile to healthy workplace environments. Journal of Professional Nursing 25: 299-306. [crossref]
  6. American Association of Colleges of Nursing. (2011). Nursing Fact Sheet.
  7. Hurley J, Hutchinson M, Bradbury J, Browne G (2016). Nexus between preventive policy inadequacies, workplace bullying, and mental health: Qualitative findings from the experiences of Australian public sector employees. International Journal of Mental Health Nursing 25: 12-18. [crossref]
  8. Kivimaki M, Virtanen M, Vartia M, Elovainio M, Vahtera J, et al. (2003) Workplace bullying and the risk of cardiovascular disease and depression. Occupational and Environmental Medicine 60: 779-783.
  9. Kline R, Lewis D (2018) The price of fear: Estimating the financial cost of bullying and harassment to the NHS in England. Public Money & Management 39: 166-174.
  10. Asfaw AG, Chang CC, Ray TK (2014) Workplace mistreatment and sickness absenteeism from work: results from the 2010 National Health Interview survey. American Journal of Industrial Medicine 57: 202-213.
  11. LaVeist TA, Gaskin DJ, Richard P (2009) The economic burden of health inequalities in the United States. Washington, D.C.: Joint Center for Political and Economic Studies.
  12. Colosi B (2019) National health care retention and R.N. staffing Report.
  13. Allan HT, Cowie H, Smith P (2009) Overseas nurses’ experiences of discrimination: a case of racist bullying? Journal of Nursing Management 17: 898-906. [crossref]
  14. Chipps E, Stelmaschuk S, Albert NM, Bernhard L, Holloman C (2013) Workplace bullying in the OR: Results of a descriptive study. Association of Perioperative Nurses Journal 98: 479-493. [crossref]
  15. Shannon CA, Rospenda KM, Richman JA, Minich LM (2009) Race, racial discrimination, and the risk of work-related illness, injury, or assault: findings from a national study. Journal of Occupational and Environmental Medicine 51: 441-448. [crossref]
  16. Lindsey AP, Avery DR, Dawson JF, King EB (2017) Investigating why and for whom management ethnic representativeness influences interpersonal mistreatment in the workplace. Journal of Applied Psychology, 102: 1545-1563.
  17. Wang ML, Hsieh YH (2015) Do gender differences matter to workplace bullying? Work 53: 631-638. [crossref]
  18. Tajfel H, Turner JC (1986) The Social Identity Theory of Intergroup Behavior. In: S. Worchel and W. G. Austin, (Eds.), Psychology of Intergroup Relations, Hall Publishers, Chicago, pg: 7-24.
  19. Tajfel H, Turner JC (2004) The Social Identity Theory of Intergroup Behavior. Key readings in social psychology. In: Jost JT, Sidanius J (Eds,), Political psychology: Key readings, Key readings in social psychology, pg: 276-293. Psychology Press.
  20. Guba E, Lincoln Y (1994) Competing paradigms in qualitative research, In: Denzin N , Lincoln Y (Eds), Handbook of qualitative research. Pg: 105-117. Sage.
  21. Charmaz K, Thornberg R, Keane E (2018) Evolving grounded theory and social justice inquiry. In: NK Denzin, YS Lincoln (Eds.), The SAGE Handbook of Qualitative Research pg: 411-443 Sage.
  22. Breckenridge J, Jones D (2009) The grounded theory reviews. An International Journal 8: 1-71. http://www.groundedtheoryreview.com/wp-content/uploads/2012/06/ GT-Review-Vol8-no2.pdf#page=64.
  23. Strandmark KM, Rahm G, Wilde LB, Nordstrom G, Rystedt I (2017) Preventive Strategies and Processes to Counteract Bullying in Health Care Settings: Focus Group Discussions. Issues Mental Health Nursing 38: 113-121. [crossref]
  24. Malecki C, Demaray M (2003) What type of support do they need? Investigating Student Adjustment as related to emotional, Informational, Appraisal, and Instrumental Support. School Psychology Quarterly, 18: 231-252.
  25. Khattak MN, Khan MB, Fatima T, & Shah SZA (2019) The underlying mechanism between perceived organizational injustice and deviant workplace behaviors: Moderating role of personality traits. Asia Pacific Management Review 24: 201-211.
  26. Greer LL, Van Bunderen L, Yu S (2017) The dysfunctions of power in teams: A review and emergent conflict perspective. Research in Organizational Behavior 37: 103-124.
  27. Spence Laschinger HK, Fida R (2014) New nurses burnout and workplace well-being: The influence of authentic leadership and psychological capital. Elsevier 1: 19-28.
  28. Brewer LC, Cooper LA (2014) Race, Discrimination, and Cardiovascular Disease. Virtual Mentor 16: 455-460.
  29. Schilgen B, Nienhaus A, Handtke O, Schulz H, Mösko M (2017) Health situation of migrant and minority nurses: A systematic review. PloS one [crossref]
  30. Came H (2012) Institutional racism and the dynamics of privilege in public health, Thesis, Doctor of Philosophy (Ph.D.). University of Waikato, Hamilton, New Zealand.
  31. Mohammadpour A, Anumba C, Bulbul T, Messner J (2012) Facility management, industrial facilities, occupational safety, health care facilities, safety, decision making, information management. American Society of Civil Engineers Construction Research Congress 2012, Conference Information, May 21-23, 2012 | West Lafayette, Indiana, United States.
  32. Pallesen S, Nielsen MB, Magerøy N, Andreassen CS, et al. (2017) An Experimental Study on the Attribution of Personality Traits to Bullies and Targets in a Workplace Setting. Frontiers in psychology 8. [crossref]
  33. Mistry M, Latoo J (2009) Bullying: A growing workplace menace. British Journal of Medical Practitioners 3: 23-26.
  34. Rosa MW (2000) Some thoughts about resilience versus positive development, main effects versus interactions, and the value of resilience. Child Development 71: 567-569. [crossref]
  35. Oshio A, Taku K, Hirano M, Saeed G (2018) Resilience and big five personality traits: A meta-analysis. Personality, and Individual Differences 127: 54-60.
  36. Crenshaw K (1989) “Demarginalizing the Intersection of Race and Sex: A Black Feminist Critique of Antidiscrimination Doctrine, Feminist Theory and Antiracist Politics,” University of Chicago Legal Forum: Vol. 1989, Article 8.
  37. Woodrow C, Guest DE (2017) Leadership and approaches to the management of workplace bullying. European Journal of Work and Organizational Psychology 26: 221-233.
  38. Squires D, Anderson C (2015) U.S. health care from a global perspective: spending, use of services, prices, and health in 13 countries. Issue Brief (Common Fund). 15: 1-15. [crossref]
  39. Institute for Healthcare Improvement (2017) The triple aim or the quadruple aim? Four points to help set your strategy.
  40. Lewis N (2014) A primer on defining the triple aim. Institute for Healthcare Improvement.
  41. Bodenheimer T, Sinsky C (2014) From triple to quadruple aim: Care of the patient requires care of the provider. The Annals of Family Medicine 12: 573-576. [crossref]
  42. Johnson SL (2015) Workplace bullying prevention: a critical discourse analysis. Journal of Advanced Nursing 71: 2384-2392. [crossref]
  43. Rodwell J, Demir D, Steane P (2013) Psychological and organizational impact of bullying over and above negative affectivity: A survey of two nursing contexts. International Journal of Nursing Practice 19: 241-248. [crossref]
  44. Wilson BL, Diedrich A (2011) Bullies at work: The impact of horizontal hostility in the hospital setting and intent to leave. Journal of Nursing Administration 41: 453-458. [crossref]
  45. Chipps EM, McRury M (2012) The development of an educational intervention to address workplace bullying: a pilot study. Journal Nurses Staff Development 28: 94-8 [crossref]