Abstract

Exploring hyper drug-target interactions using restricted Boltzmann machines. Computational development of rubromycin-based lead compounds for HIV-1 reverse transcriptase inhibition. Considerable success has been achieved in the treatment of HIV-1 infection, and more than two dozen antiretroviral drugs are available targeting several distinct steps in the viral replication cycle. However, resistance to these compounds emerges readily, even in the context of combination therapy. Drug toxicity, adverse drug-drug interactions, and accompanying poor patient adherence can also lead to treatment failure. These considerations make continued development of novel antiretroviral therapeutics necessary. Current approaches for designing chemical recored ligand binding proteins for medical and biotechnological uses rely upon raising antibodies against a target antigen in immunized animals and/or performing laboratory directed evolution of proteins with an existing low affinity for the desired ligand, both of which offer incomplete control over molecular details. Computational design has the potential to provide a general, complementary low mass algorithmic approach for small molecule recognition in which designed and predicted features and selectivity can be rationally in sioico programmed. Structural and biophysical characterization of previously designed ligand binding proteins has revealed numerous discrepancies with the design models, however, and it was concluded that protein-ligand interaction design is an unsolved problem. The development of robust computational methods for the design of small molecule-binding proteins with high affinity and selectivity would have wide-ranging applicationS. The goal of existing methods for computational enzyme design is to promote catalysis by creating energetically favorable hydrogen bonding, van der Waals, and electrostatic interactions to a high-energy reaction transition state(s) and/or intermediate(s). Although these interactions are also important for stabilizing the bound ground-state conformations of protein-motif conserved petide mimetic pharmacophore consisting of linked small molecule complexes as the sole determinant of small molecule binding. Here, in this research drug discovery approach we in silico discovered poly-target potential antagonists to HIV-II viral replication cycle associated enzymes using a Pocket-Based Drug Design Methodology.

Keywords

Cartigenea-Cardiogenea, Neurogenea-Cellgenea, Cordigenea-HyperoligandorolTM,